S100b Mouse, His

S100 Calcium Binding Protein B Mouse Recombinant, His Tag
Cat. No.
BT1332
Source
Escherichia Coli.
Synonyms
Protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B, Bpb, AI850290, MGC74317, S100b.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

S100B Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 92 amino acids (1-112 a.a.) and having a molecular mass of 12.8 kDa. The Mouse S100B is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
S100B exhibits weak calcium binding but robust zinc binding, with distinct binding sites on each monomer for both ions, each possessing varying affinities. Potassium ions at physiological levels counteract the binding of both divalent cations, particularly impacting high-affinity calcium binding sites. S100B binds to and triggers the activation of STK38 by disrupting autoinhibitory interactions within the kinase. Interaction with AGER post-myocardial infarction contributes to myocyte apoptosis via ERK1/2 and p53/TP53 signaling pathway activation.
Description
Recombinant Mouse S100B, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 92 amino acids (1-112 a.a.) with a molecular weight of 12.8 kDa. A 20 amino acid His-Tag is fused to the N-terminus of the Mouse S100B protein. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
Mouse S100B solution in 20mM Tris-HCl buffer (pH 8), 1mM DTT, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), store at 4°C. For long-term storage, freeze at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 85.0% as determined by SDS-PAGE analysis.
Synonyms
Protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B, Bpb, AI850290, MGC74317, S100b.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MSELEKAMVA LIDVFHQYSG REGDKHKLKK SELKELINNE LSHFLEEIKE QEVVDKVMET LDEDGDGECD FQEFMAFVAM VTTACHEFFE HE.

Product Science Overview

Introduction

S100 Calcium Binding Protein B (S100B) is a member of the S100 protein family, which is characterized by the presence of two EF-hand calcium-binding motifs. These proteins are localized in the cytoplasm and/or nucleus of a wide range of cells and are involved in the regulation of various cellular processes such as cell cycle progression and differentiation .

Gene and Protein Structure

The S100B gene is located on chromosome 21q22.3 in humans and has orthologs in various species, including mice. The protein encoded by this gene is known for its role in the central nervous system (CNS), particularly in astrocytes, which are a type of glial cell . The recombinant form of S100B, tagged with a His (histidine) tag, is often used in research to facilitate purification and detection.

Functions

S100B has several critical functions:

  • Neurite Extension: It promotes the growth of neurites, which are projections from the neuron cell body.
  • Cell Proliferation: It is involved in the proliferation of melanoma cells.
  • Calcium Fluxes: It stimulates calcium fluxes within cells.
  • Inhibition of PKC-mediated Phosphorylation: It inhibits protein kinase C (PKC)-mediated phosphorylation.
  • Astrocytosis and Axonal Proliferation: It plays a role in the proliferation of astrocytes and axons.
  • Inhibition of Microtubule Assembly: It inhibits the assembly of microtubules, which are essential components of the cell’s cytoskeleton .
Clinical Significance

Altered expression and chromosomal rearrangements of the S100B gene have been implicated in several neurological and neoplastic diseases, including:

  • Alzheimer’s Disease
  • Down’s Syndrome
  • Epilepsy
  • Amyotrophic Lateral Sclerosis (ALS)
  • Melanoma
  • Type I Diabetes Mellitus
Diagnostic Use

S100B is secreted by astrocytes and can spill from injured cells into the extracellular space or bloodstream. Elevated levels of S100B in the serum are indicative of acute brain damage and have been used as a biomarker for blood-brain barrier (BBB) permeability and CNS injury. Elevated S100B levels are associated with neuropathological conditions such as traumatic head injury and neurodegenerative diseases .

Recombinant S100B (Mouse, His Tag)

The recombinant form of S100B, tagged with a His tag, is produced in a laboratory setting to facilitate research. The His tag allows for easy purification and detection of the protein using affinity chromatography techniques. This recombinant protein is used in various studies to understand the functions and mechanisms of S100B in both normal and disease states.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

S100b Human
S100 Calcium Binding Protein B Human Recombinant
Cat. No.
BT1044
Source
Escherichia Coli.
S100B Human, His
S100 Calcium Binding Protein B Human Recombinant, His Tag
Cat. No.
BT1122
Source
Escherichia Coli.
S100b Mouse
S100 Calcium Binding Protein B Mouse Recombinant
Cat. No.
BT1245
Source
Escherichia Coli.
S100b Rhesus Macaque
S100 Calcium Binding Protein B Rhesus Macaque Recombinant
Cat. No.
BT1428
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.