S100B Human, His

S100 Calcium Binding Protein B Human Recombinant, His Tag
Cat. No.
BT1122
Source
Escherichia Coli.
Synonyms
Protein S100-B, S100 calcium-binding protein B, S-100 protein subunit beta, S-100 protein beta chain, S100B, NEF, S100, S100beta.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

S100B Human Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 112 amino acids fragment (1-92) with a 20 amino acids N-terminal His tag and having a total molecular mass of 12.8kDa.
The S100B is purified by proprietary chromatographic techniques.

Product Specs

Introduction
S100B is a protein belonging to the S100 family, a group of EF-hand calcium-binding proteins typically found as dimers. The S100B homodimer is present in cells of the central nervous system, glial cells, and specific peripheral cells like Schwann cells, melanocytes, adipocytes, and chondrocytes. S100 proteins are located in either the cytoplasm or nucleus of various cells and participate in regulating cellular processes such as cell cycle progression and differentiation. While at least 13 members of the S100 gene family cluster on chromosome 1q21, S100b is located at 21q22.3. Measuring serum S100B levels can help monitor brain injury and malignant melanoma. S100b proteins may be involved in neurite extension, melanoma cell proliferation, stimulation of calcium ion fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Alterations in S100b gene expression and chromosomal rearrangements are implicated in various neurological, neoplastic, and other diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.
Description
Recombinant human S100B, produced in E. coli, is a non-glycosylated polypeptide chain containing 112 amino acids. This includes an S100B fragment (amino acids 1-92) with a 20 amino acid N-terminal His tag. The total molecular mass is 12.8kDa. S100B is purified using proprietary chromatographic techniques.
Physical Appearance
A clear solution, sterilized by filtration.
Formulation
S100B is supplied at a concentration of 1mg/ml in a buffer solution containing 20mM Tris-HCl (pH 8.0), 1mM DTT, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, freezing at -20°C is recommended. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freezing and thawing should be avoided.
Purity
Purity is determined to be greater than 90.0% using SDS-PAGE analysis.
Synonyms
Protein S100-B, S100 calcium-binding protein B, S-100 protein subunit beta, S-100 protein beta chain, S100B, NEF, S100, S100beta.
Source
Escherichia Coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MSELEKAMVA LIDVFHQYSG REGDKHKLKK SELKELINNE LSHFLEEIKE QEVVDKVMET LDNDGDGECD FQEFMAFVAM VTTACHEFFE HE.

Product Science Overview

Introduction

S100 Calcium Binding Protein B (S100B) is a member of the S100 family of proteins, which are characterized by their ability to bind calcium through EF-hand motifs. These proteins are involved in a variety of intracellular and extracellular functions, including regulation of protein phosphorylation, enzyme activities, cell growth, and differentiation .

Structure and Function

S100B is a homodimeric protein, meaning it consists of two identical subunits. Each subunit contains two EF-hand calcium-binding motifs. The protein is primarily found in the cytoplasm and nucleus of astrocytes and certain other cell types .

The primary functions of S100B include:

  • Neurite extension: Promoting the growth of nerve cell projections.
  • Proliferation of melanoma cells: Stimulating the growth of melanoma cancer cells.
  • Regulation of calcium fluxes: Modulating the movement of calcium ions within cells.
  • Inhibition of protein kinase C (PKC) phosphorylation: Affecting various signaling pathways.
  • Astrocytosis and axonal proliferation: Involvement in the response of astrocytes to injury and the growth of axons.
  • Inhibition of microtubule assembly: Affecting the structural components of cells .
Recombinant S100B with His Tag

Recombinant S100B proteins are produced using genetic engineering techniques, where the S100B gene is inserted into an expression system, such as E. coli, to produce the protein in large quantities. The “His Tag” refers to a sequence of histidine residues added to the N-terminus of the protein. This tag facilitates the purification of the protein using affinity chromatography, as histidine residues bind strongly to nickel ions .

Applications

Recombinant S100B proteins are widely used in research to study their role in various cellular processes and diseases. Some key applications include:

  • Functional assays: Measuring the ability of S100B to bind to other proteins, such as AGER and TP53, in ELISA assays .
  • Disease research: Investigating the role of S100B in conditions like Alzheimer’s disease, Down’s syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes .
  • Biomarker studies: Using S100B as a marker for brain injury and other neurological conditions .

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