RGS16 Human

Regulator of G-Protein Signaling 16 Human Recombinant
Cat. No.
BT12602
Source
Escherichia Coli.
Synonyms
A28-RGS14, A28-RGS14P, RGS-R, Regulator of G-protein signaling 16, RGS16, Retinally abundant regulator of G-protein signaling, Retinal-specific RGS, hRGS-r, RGSR.
Appearance
Sterile Filtered clear colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

RGS16 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 222 amino acids (1-202 a.a.) and having a molecular mass of 24.9 kDa. The RGS16 is fused to a 20 amino acid His Tag at N-terminal and purified by proprietary chromatographic techniques.

Product Specs

Introduction
RGS16, a member of the regulator of G protein signaling family, functions by accelerating the GTPase activity of G protein alpha subunits, converting them into their inactive GDP-bound state. It exhibits specific binding to G(i)-alpha and G(o)-alpha, but not G(s)-alpha. Notably, RGS16 plays a crucial role in modulating the signaling kinetics within the phototransduction cascade.
Description
Recombinant human RGS16, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 222 amino acids (residues 1-202) with a molecular weight of 24.9 kDa. A 20 amino acid His Tag is fused to the N-terminus of the protein, which is purified using proprietary chromatographic methods.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The RGS16 solution is provided at a concentration of 0.5 mg/ml in Phosphate Buffered Saline (pH 7.4), 0.1M NaCl, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), the solution should be kept at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freeze-thaw cycles should be avoided.
Purity
The purity of the RGS16 protein is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
A28-RGS14, A28-RGS14P, RGS-R, Regulator of G-protein signaling 16, RGS16, Retinally abundant regulator of G-protein signaling, Retinal-specific RGS, hRGS-r, RGSR.
Source
Escherichia Coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MCRTLAAFPT TCLERAKEFK TRLGIFLHKS ELGCDTGSTG KFEWGSKHSK ENRNFSEDVL GWRESFDLLL SSKNGVAAFH AFLKTEFSEE NLEFWLACEE FKKIRSATKL ASRAHQIFEE FICSEAPKEV NIDHETRELT RMNLQTATAT CFDAAQGKTR TLMEKDSYPR FLKSPAYRDL AAQASAASAT LSSCSLDEPS HT.

Product Science Overview

Introduction

The Regulator of G-Protein Signaling 16 (RGS16) is a member of the RGS protein family, which plays a crucial role in the modulation of G-protein-coupled receptor (GPCR) signaling pathways. These pathways are essential for various physiological processes, including immune response, inflammation, and circadian rhythm regulation.

Structure and Function

RGS16 belongs to the small B/R4 subfamily of RGS proteins. It consists of a conserved RGS structural domain with short, disordered amino- and carboxy-terminal extensions and an α-helix that binds and deactivates heterotrimeric G proteins . The primary function of RGS16 is to act as a guanosine triphosphatase (GTPase) activating protein (GAP), accelerating the hydrolysis of GTP to GDP on the Gα subunit of G proteins. This action leads to the termination of GPCR signaling .

Role in Circadian Rhythm

RGS16 is significantly involved in the regulation of circadian rhythms. It is expressed in the suprachiasmatic nucleus (SCN) of the brain, which is the central circadian clock. RGS16, along with the orphan receptor GPR176, regulates the synthesis of cyclic adenosine monophosphate (cAMP) in the SCN, thereby influencing the pace of the circadian clock .

Involvement in Immune Response and Inflammation

RGS16 also plays a vital role in immune response and inflammation. It regulates various signaling pathways, including the mitogen-activated protein kinase (MAPK), phosphoinositide 3-kinase/protein kinase B (PI3K/Akt), Ras homolog family member A (RhoA), and stromal cell-derived factor 1/C-X-C motif chemokine receptor 4 (SDF-1/CXCR4) pathways . These pathways are crucial for immune cell activation, migration, and cytokine production.

Implications in Diseases

Given its regulatory functions, RGS16 is implicated in several diseases. It is involved in the inflammatory response induced by the Hepatitis B Virus (HBV) and has been linked to various cancers and metabolic disorders . Understanding the role of RGS16 in these diseases can provide insights into potential therapeutic targets.

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