RGS10 Human

Regulator of G-Protein Signaling 10 Human Recombinant
Cat. No.
BT12451
Source
E.coli.
Synonyms
Regulator Of G-Protein Signaling 10, RGS10.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

RGS10 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 205 amino acids (1-181) and having a molecular mass of 23.7 kDa.
The RGS10 is fused to a 24 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
RGS10, a member of the RGS protein family, plays a crucial role in regulating G protein signaling. It functions as a GTPase-activating protein (GAP) for G alpha subunits of heterotrimeric G proteins, particularly Gi alpha, Go alpha, and Gq alpha subtypes. By enhancing the GTPase activity of these G alpha subunits, RGS10 promotes their conversion to the inactive GDP-bound state. This regulatory action is mediated by the conserved 120-amino acid RGS domain present in all RGS proteins. Notably, RGS10 exhibits specific binding to the activated forms of G-alphai3 and G-alphaz, both of which are related G protein subunits. However, it does not interact with structurally and functionally distinct G-alpha subunits. The localization of RGS10 within the nucleus further suggests its involvement in modulating nuclear G protein signaling pathways.
Description
Recombinant RGS10, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 205 amino acids (residues 1-181). With a molecular weight of 23.7 kDa, it features a 24 amino acid His-Tag fused to its N-terminus. This protein has been purified using proprietary chromatographic techniques to ensure its high quality and purity.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The RGS10 solution is provided at a concentration of 1 mg/ml. It is formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 M NaCl, and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the RGS10 vial can be stored at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. Adding a carrier protein like HSA or BSA (0.1%) can further enhance stability during long-term storage. To maintain the integrity of the protein, it is essential to avoid repeated cycles of freezing and thawing.
Purity
The purity of RGS10 is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
Regulator Of G-Protein Signaling 10, RGS10.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMFNRAV SRLSRKRPPS DIHDSDGSSS SSHQSLKSTA KWAASLENLL EDPEGVKRFR EFLKKEFSEE NVLFWLACED FKKMQDKTQM QEKAKEIYMT FLSSKASSQV NVEGQSRLNE KILEEPHPLM FQKLQDQIFN LMKYDSYSRF LKSDLFLKHK RTEEEEEDLP DAQTAAKRAS RIYNT

Product Science Overview

Introduction

The Regulator of G-Protein Signaling 10 (RGS10) is a member of the RGS family, which plays a crucial role in the regulation of G-protein-coupled receptor (GPCR) signaling pathways. These pathways are essential for various physiological processes, including sensory perception, immune responses, and neurotransmission.

Gene and Protein Structure

The RGS10 gene is located on chromosome 10q26.11 and spans approximately 35 kb, containing five exons . The gene encodes a protein that is 173 amino acids long and has a molecular weight of approximately 20 kDa . The RGS10 protein contains a conserved 120-amino acid RGS domain, which is essential for its function as a GTPase-activating protein (GAP) .

Function

RGS10 negatively regulates GPCR signaling by increasing the GTPase activity of G-protein alpha subunits, driving them into their inactive GDP-bound form . This regulation is crucial for maintaining the balance and proper functioning of cellular signaling pathways. RGS10 specifically interacts with the activated forms of G-alphai3 and G-alphaz subunits but does not interact with other G-alpha subunits .

Expression and Localization

RGS10 is expressed in various tissues, including the brain, liver, kidney, and certain cell lines . It is highly expressed in osteoclasts and preosteoclasts, indicating its role in bone metabolism . The protein is localized in the nucleus, suggesting it may have additional regulatory functions within the cell .

Clinical Significance

Mutations or dysregulation of RGS10 have been associated with several diseases, including leukoencephalopathy with vanishing white matter and epilepsy . Understanding the role of RGS10 in these conditions could provide insights into potential therapeutic targets.

Recombinant RGS10

Recombinant human RGS10 is produced using advanced biotechnological methods to ensure high purity and bioactivity . This recombinant protein is used in various research applications to study its function and role in cellular signaling pathways.

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