RGS14 Human
Regulator of G-Protein Signaling 14 Human Recombinant
Cat. No.
BT12530
Source
Escherichia Coli.
Synonyms
Regulator of G-protein signaling 14, RGS14.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 80% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
RGS14 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 586 amino acids (1-566 a.a) and having a molecular mass of 63.6kDa.
RGS14 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
RGS14 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
RGS14, or Regulator of G-protein signaling 14, belongs to the regulator of G protein signaling family. This protein is characterized by its RGS domain, two Raf-like Ras-binding domains (RBDs), and a GoLoco motif. Functionally, RGS14 modulates G-protein signaling activity. It achieves this by employing its GoLoco domain to bind to specific activated, GTP-bound G alpha subunits. Acting as a scaffolding protein, RGS14 integrates G protein and H-Ras/ERK/MAP kinase signaling pathways. This strategic positioning allows it to control plasticity in CA2 neurons. Given its high concentration in CA2 pyramidal neurons, RGS14 plays a crucial role in regulating synaptic plasticity at these synapses and influencing hippocampal-based learning and memory.
Description
Recombinant Human RGS14, produced in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 586 amino acids (specifically, amino acids 1-566) and exhibits a molecular weight of 63.6kDa. The protein is engineered with a 20 amino acid His-tag at its N-terminus and purified using specialized chromatographic techniques.
Physical Appearance
The product appears as a clear, colorless solution that has been sterilized by filtration.
Formulation
The RGS14 protein is provided in a solution at a concentration of 0.5mg/ml. The solution is buffered with 20mM Tris-HCl at a pH of 7.5 and contains 10% glycerol, 1mM DTT, and 200mM NaCl.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the product frozen at -20°C. To ensure optimal long-term stability, consider adding a carrier protein such as HSA or BSA (at a concentration of 0.1%). It's important to avoid repeated freeze-thaw cycles to maintain product integrity.
Purity
The purity of the RGS14 protein is determined to be greater than 80% based on SDS-PAGE analysis.
Synonyms
Regulator of G-protein signaling 14, RGS14.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MPGKPKHLGV PNGRMVLAVS DGELSSTTGP QGQGEGRGSS LSIHSLPSGP SSPFPTEEQP VASWALSFER LLQDPLGLAY FTEFLKKEFS AENVTFWKAC ERFQQIPASD TQQLAQEARN IYQEFLSSQA LSPVNIDRQA WLGEEVLAEP RPDMFRAQQL QIFNLMKFDS YARFVKSPLY RECLLAEAEG RPLREPGSSR LGSPDATRKK PKLKPGKSLP LGVEELGQLP PVEGPGGRPL RKSFRRELGG TANAALRRES QGSLNSSASL DLGFLAFVSS KSESHRKSLG STEGESESRP GKYCCVYLPD GTASLALARP GLTIRDMLAG ICEKRGLSLP
DIKVYLVGNE QALVLDQDCT VLADQEVRLE NRITFELELT ALERVVRISA KPTKRLQEAL QPILEKHGLS PLEVVLHRPG EKQPLDLGKL VSSVAAQRLV LDTLPGVKIS KARDKSPCRS QGCPPRTQDK ATHPPPASPS SLVKVPSSAT GKRQTCDIEG LVELLNRVQS SGAHDQRGLL
RKEDLVLPEF LQLPAQGPSS EETPPQTKSA AQPIGGSLNS TTDSAL.
DIKVYLVGNE QALVLDQDCT VLADQEVRLE NRITFELELT ALERVVRISA KPTKRLQEAL QPILEKHGLS PLEVVLHRPG EKQPLDLGKL VSSVAAQRLV LDTLPGVKIS KARDKSPCRS QGCPPRTQDK ATHPPPASPS SLVKVPSSAT GKRQTCDIEG LVELLNRVQS SGAHDQRGLL
RKEDLVLPEF LQLPAQGPSS EETPPQTKSA AQPIGGSLNS TTDSAL.
Product Science Overview
Introduction
Regulator of G-Protein Signaling 14 (RGS14) is a multifunctional protein that plays a crucial role in the regulation of G-protein signaling pathways. It is a member of the RGS protein family, which is known for its ability to accelerate the GTPase activity of Gα subunits, thereby terminating G-protein-coupled receptor (GPCR) signaling. RGS14 is encoded by the RGS14 gene in humans and has been extensively studied for its diverse functions and interactions.
Structure and Domains
RGS14 contains several distinct domains that contribute to its multifunctionality:
- RGS Domain: This domain is responsible for its GTPase-activating protein (GAP) activity, which accelerates the hydrolysis of GTP to GDP on Gα subunits, leading to the inactivation of G-proteins .
- Raf-like Ras-binding Domains (RBDs): RGS14 has two tandem Ras-binding domains that allow it to interact with members of the Ras and Rap families of small GTPases .
- GoLoco Motif: This motif enables RGS14 to bind to specific Gα subunits in their GDP-bound state, further regulating G-protein signaling .
Functions and Mechanisms
RGS14 is involved in various cellular processes through its interactions with different signaling molecules:
- G-Protein Regulation: By acting as a GAP, RGS14 accelerates the deactivation of Gα subunits, thereby modulating GPCR signaling pathways .
- Ras/Rap Signaling: RGS14 can bind to both Ras and Rap GTPases, although it has been shown to selectively interact with activated H-Ras in cellular contexts . This interaction facilitates the formation of a multiprotein complex that promotes sustained ERK activation, which is essential for processes like neuronal differentiation .
- Neuronal Functions: RGS14 has been implicated in the regulation of synaptic plasticity and memory. It is enriched in specific regions of the brain, such as the CA2 pyramidal neurons, where it suppresses synaptic plasticity and hippocampal-based learning .
Research and Implications
Research on RGS14 has revealed its potential implications in various physiological and pathological conditions:
- Memory and Learning: Studies have shown that increasing the expression of RGS14 in certain brain regions can enhance the conversion of short-term to long-term memory .
- Neuronal Differentiation: RGS14 plays a role in neuronal differentiation by facilitating the formation of signaling complexes that promote ERK activation .
- Disease Associations: Dysregulation of RGS14 has been linked to various neurological disorders, highlighting its importance in maintaining normal brain function .
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