RBM8A Human

RNA Binding Motif Protein 8A Human Recombinant
Cat. No.
BT19016
Source
Escherichia Coli.
Synonyms
RNA-binding protein 8A, Binder of OVCA1-1, BOV-1, RNA-binding motif protein 8A, RNA-binding protein Y14, Ribonucleoprotein RBM8A, RBM8A, RBM8, Y14, ZNRP, RBM8B, ZRNP1, BOV-1A, BOV-1B, BOV-1C, MDS014.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

RBM8A produced in E.Coli is a single, non-glycosylated polypeptide chain containing 182 amino acids (1-174 a.a.) and having a molecular mass of 20.9kDa.
RBM8A is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
RBM8A, a core component of the exon junction complex (EJC), plays a crucial role in mRNA metabolism. The EJC participates in mRNA export from the nucleus, localization within the cytoplasm, and the process of nonsense-mediated mRNA decay (NMD). RBM8A acts as a messenger, conveying information about the mRNA's processing history, such as the location of removed introns, to the cytoplasm. While RBM8A is capable of shuttling between the nucleus and cytoplasm, it primarily resides in the nucleus and co-localizes with oskar mRNA at the posterior pole of the cell.
Description
Recombinant RBM8A, expressed in E. coli, is a single, non-glycosylated polypeptide chain encompassing 182 amino acids (residues 1-174) with a molecular weight of 20.9 kDa. This protein includes an 8-amino acid His-tag fused at its C-terminus and undergoes purification using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The RBM8A solution is provided at a concentration of 0.5 mg/ml in a buffer composed of 20 mM Tris-HCl (pH 8.0), 10% glycerol, 0.1 M NaCl, and 1 mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the RBM8A solution should be kept refrigerated at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. To ensure optimal stability during long-term storage, the addition of a carrier protein such as 0.1% HSA or BSA is advised. It is important to minimize repeated freeze-thaw cycles to maintain protein integrity.
Purity
The purity of RBM8A is determined to be greater than 90% as assessed by SDS-PAGE analysis.
Synonyms
RNA-binding protein 8A, Binder of OVCA1-1, BOV-1, RNA-binding motif protein 8A, RNA-binding protein Y14, Ribonucleoprotein RBM8A, RBM8A, RBM8, Y14, ZNRP, RBM8B, ZRNP1, BOV-1A, BOV-1B, BOV-1C, MDS014.
Source
Escherichia Coli.
Amino Acid Sequence
MADVLDLHEA GGEDFAMDED GDESIHKLKE KAKKRKGRGF GSEEGSRARM REDYDSVEQD GDEPGPQRSV EGWILFVTGV HEEATEEDIH DKFAEYGEIK NIHLNLDRRT GYLKGYTLVE YETYKEAQAA MEGLNGQDLM GQPISVDWCF VRGPPKGKRR GGRRRSRSPD RRRRLEHHHH HH.

Product Science Overview

Gene and Protein Structure

The RBM8A gene is located on chromosome 1 at the 1q21.1 region . It encodes a protein with a conserved RNA-binding motif, which is essential for its function in RNA metabolism. The protein is predominantly found in the nucleus but is also present in the cytoplasm .

Function and Mechanism

RBM8A is preferentially associated with mRNAs produced by splicing, including both nuclear mRNAs and newly exported cytoplasmic mRNAs . It is thought to remain associated with spliced mRNAs as a tag to indicate where introns had been present, thus coupling pre- and post-mRNA splicing events . This tagging mechanism is crucial for the proper processing and transport of mRNA within the cell.

Biological Processes

RBM8A is involved in several important biological processes, including:

  • mRNA Splicing: It is a core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs .
  • mRNA Transport: It helps in the transport of mRNA from the nucleus to the cytoplasm .
  • Regulation of Translation: It plays a role in the regulation of translation, ensuring that mRNAs are correctly processed and translated into proteins .
Clinical Significance

Mutations in the RBM8A gene are associated with several genetic disorders. One notable condition is Thrombocytopenia-Absent Radius (TAR) Syndrome, which is characterized by the absence of the radius bone in the forearm and a low platelet count . This syndrome is caused by an allele mutation and a low-frequency noncoding single-nucleotide polymorphism (SNP) in the RBM8A gene .

Research and Applications

The recombinant form of RBM8A is used in various research applications to study its function and role in RNA metabolism. Understanding the mechanisms by which RBM8A operates can provide insights into the regulation of gene expression and the development of genetic disorders.

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