RBM17 Human

RNA Binding Motif Protein 17 Human Recombinant
Cat. No.
BT18748
Source
Escherichia Coli.
Synonyms
RNA Binding Motif Protein 17, SPF45, Splicing Factor 45kDa, RNA-Binding Motif Protein 17, Splicing Factor 45, 45 kDa-splicing factor, RBM17.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

RBM17 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 421 amino acids (1-401 a.a) and having a molecular mass of 47.1kDa.
RBM17 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
RNA binding motif protein 17 (RBM17) is a splice factor that binds to the single-stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. RBM17 is involved in regulating alternative splicing and the utilization of cryptic splice sites. It also stimulates the utilization of a cryptic splice site created by the beta-110 mutation in the HBB gene. The resulting frameshift leads to sickle cell anemia.
Description
Recombinant human RBM17, produced in E. coli, is a single, non-glycosylated polypeptide chain containing 421 amino acids (1-401 a.a) and having a molecular mass of 47.1 kDa. RBM17 is fused to a 20 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, sterile, filtered solution.
Formulation
RBM17 protein solution (0.5 mg/mL) in 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 10% glycerol, and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), store at 4°C. For long-term storage, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Greater than 90.0% purity as determined by SDS-PAGE.
Synonyms
RNA Binding Motif Protein 17, SPF45, Splicing Factor 45kDa, RNA-Binding Motif Protein 17, Splicing Factor 45, 45 kDa-splicing factor, RBM17.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MSLYDDLGVE TSDSKTEGWS KNFKLLQSQL QVKKAALTQA KSQRTKQSTV LAPVIDLKRG GSSDDRQIVD TPPHVAAGLK DPVPSGFSAG EVLIPLADEY DPMFPNDYEK VVKRQREERQ RQRELERQKE IEEREKRRKD RHEASGFARR PDPDSDEDED YERERRKRSM GGAAIAPPTS LVEKDKELPR DFPYEEDSRP RSQSSKAAIP PPVYEEQDRP RSPTGPSNSF LANMGGTVAH KIMQKYGFRE GQGLGKHEQG LSTALSVEKT SKRGGKIIVG DATEKDASKK SDSNPLTEIL KCPTKVVLLR NMVGAGEVDE DLEVETKEEC EKYGKVGKCV IFEIPGAPDD EAVRIFLEFE RVESAIKAVV DLNGRYFGGR VVKACFYNLD KFRVLDLAEQ V.

Product Science Overview

Biological Properties and Expression Patterns

RBM17 is a crucial component of the spliceosome complex, which is involved in the second catalytic step of mRNA splicing . The protein binds to the single-stranded 3’AG at the exon/intron border and promotes its utilization in the splicing process . This function is essential for the regulation of alternative splicing and the utilization of cryptic splice sites .

Tissue Distribution

RBM17 is expressed in various tissues, including lymphoid tissue, bone marrow, testis, skeletal muscle, and several others . Its expression is particularly significant in tissues involved in cell proliferation, immune response, and protein ubiquitination .

Biological Functions

The primary function of RBM17 is to facilitate the splicing of pre-mRNA by promoting the utilization of specific splice sites . This role is critical for the proper processing of mRNA and the regulation of gene expression. Additionally, RBM17 is involved in the regulation of alternative splicing, which allows for the generation of multiple protein isoforms from a single gene .

Modes of Action

RBM17 interacts with various proteins within the spliceosome complex to carry out its splicing functions . It binds to the 3’AG splice site and promotes its utilization in the second catalytic step of splicing . This interaction is essential for the accurate and efficient processing of pre-mRNA.

Regulatory Mechanisms

The expression and activity of RBM17 are regulated at multiple levels, including transcriptional, post-transcriptional, and post-translational modifications . Alternative splicing of the RBM17 gene itself results in multiple transcript variants, which may have distinct functions and regulatory mechanisms .

Clinical Significance

Mutations or dysregulation of RBM17 have been associated with various diseases, including spinocerebellar ataxia 1 and autosomal dominant cerebellar ataxia . Additionally, RBM17 has been implicated in the regulation of cryptic splice sites, which can lead to conditions such as sickle cell anemia .

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