RBM3 Human

RNA Binding Motif Protein 3 Human Recombinant
Cat. No.
BT18937
Source
Escherichia Coli.
Synonyms
IS1-RNPL, RNPL, RNA-binding motif protein 3, Putative RNA-Binding Protein 3, RNA Binding Motif (RNP1, RRM) Protein 3.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

RBM3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 180 amino acids (1-157 a.a) and having a molecular mass of 19kDa.
RBM3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
RBM3 is a member of the glycine-rich RNA-binding protein family. It encodes a protein with a single RNA recognition motif (RRM) domain. This gene is induced by cold shock and low oxygen tension. A pseudogene exists on chromosome 1. Several alternative transcriptional splice variants, which encode different isoforms, have been identified. RBM3 is associated with diseases like vaccinia and cryptorchidism. Its functions include RNA binding and ribosomal large subunit binding. RBMY1J is an important paralog of this gene.
Description
Recombinant human RBM3 protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 180 amino acids (with amino acids 1-157 comprising the RBM3 protein) and has a molecular weight of 19 kDa. The RBM3 protein is fused to a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear solution that has been sterilized by filtration.
Formulation
The RBM3 protein solution has a concentration of 0.25 mg/ml. It is prepared in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.2 M NaCl, 50% glycerol, and 5 mM DTT.
Stability
The RBM3 protein solution should be stored at 4°C for up to 4 weeks. For longer-term storage, it is recommended to freeze the solution at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. It is important to avoid repeated cycles of freezing and thawing.
Purity
The purity of the RBM3 protein is greater than 95% as determined by SDS-PAGE.
Synonyms
IS1-RNPL, RNPL, RNA-binding motif protein 3, Putative RNA-Binding Protein 3, RNA Binding Motif (RNP1, RRM) Protein 3.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSSEEGK LFVGGLNFNT DEQALEDHFS SFGPISEVVV VKDRETQRSR GFGFITFTNP EHASVAMRAM NGESLDGRQI RVDHAGKSAR GTRGGGFGAH GRGRSYSRGG GDQGYGSGRY YDSRPGGYGY GYGRSRDYNG RNQGGYDRYS GGNYRDNYDN

Product Science Overview

Gene and Protein Structure

The RBM3 gene is located on the X chromosome at position Xp11.23 . This gene encodes a protein that is involved in mRNA biogenesis and has anti-apoptotic effects . The protein is composed of several domains, including the RNA recognition motif, which is essential for its RNA-binding capability .

Expression and Regulation

RBM3 is known to be induced by cold shock and low oxygen tension . This induction is part of the cellular response to stress, allowing cells to adapt to harsh conditions. The expression of RBM3 is regulated at the transcriptional level, and multiple alternatively spliced transcript variants have been identified .

Functional Significance

RBM3 enhances global protein synthesis at both physiological and mild hypothermic temperatures . It reduces the relative abundance of microRNAs when overexpressed and enhances the phosphorylation of translation initiation factors, leading to active polysome formation . These functions are crucial for maintaining cellular homeostasis and promoting cell survival under stress conditions.

Clinical Significance

RBM3 has been identified as a proto-oncogene associated with tumor progression and metastasis . High levels of RBM3 protein in tumor cells have been linked to favorable prognostic outcomes in colorectal cancer . This makes RBM3 a potential biomarker for cancer diagnosis and prognosis.

Research and Applications

Human recombinant RBM3 is used in various research applications to study its role in cellular processes and its potential as a therapeutic target. The recombinant protein is produced using advanced biotechnological methods to ensure high purity and activity.

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