RAET1E Human, Sf9
Retinoic Acid Early Transcript 1E, Lymphocyte Effector Toxicity Activation Ligand, RAE-1-Like Transcript 4, NKG2DL4, N2DL-4, LETAL, ULBP4, RL-4, NKG2D Ligand 4, BA350J20.7, RAET1E2, N2DL4.
Greater than 90.0% as determined by SDS-PAGE.
Description
RAET1E Human Recombinant produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 204 amino acids (31-225 a.a.) and having a molecular mass of 23.4kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa).
RAET1E is expressed with a 6 amino acids His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Belonging to the MHC class I family, RAET1E is encoded by a gene located within the MHC class I-related gene cluster on chromosome 6q24.2-q25.3. Unlike other RAET1 proteins that possess glycosylphosphatidylinositol anchor sequences, RAET1E and RAET1G have type I membrane-spanning sequences at their C-termini. Functioning as a ligand for the NKG2D receptor, which is present on various immune cells, RAET1E participates in both innate and adaptive immune responses. By promoting the expansion of anti-tumor cytotoxic lymphocytes, RAET1E transmits signals to NK cells and contributes to tumor immune surveillance.
Recombinant human RAET1E, produced in Sf9 insect cells, is a single, glycosylated polypeptide chain consisting of 204 amino acids (residues 31-225). With a molecular weight of 23.4 kDa, it appears as a band at approximately 28-40 kDa on SDS-PAGE. The protein is expressed with a C-terminal 6-His tag and purified using proprietary chromatographic methods.
The RAET1E protein is supplied in a solution at a concentration of 0.5 mg/mL, containing Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the protein at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Multiple freeze-thaw cycles should be avoided.
The purity of RAET1E is determined to be greater than 90% using SDS-PAGE analysis.
Retinoic Acid Early Transcript 1E, Lymphocyte Effector Toxicity Activation Ligand, RAE-1-Like Transcript 4, NKG2DL4, N2DL-4, LETAL, ULBP4, RL-4, NKG2D Ligand 4, BA350J20.7, RAET1E2, N2DL4.
ADPHSLCFNF TIKSLSRPGQ PWCEAQVFLN KNLFLQYNSD NNMVKPLGLL GKKVYATSTW GELTQTLGEV GRDLRMLLCD IKPQIKTSDP STLQVEMFCQ REAERCTGAS WQFATNGEKS LLFDAMNMTW TVINHEASKI KETWKKDRGL EKYFRKLSKG DCDHWLREFL GHWEAMPEPT VSPVNASDIH WSSSSLPDHH HHHH.
Product Science Overview
RAET1E functions as a stress-induced ligand for the NKG2D receptor, which is involved in the activation of natural killer (NK) cells and certain T cells . This interaction plays a crucial role in the immune response, particularly in the recognition and elimination of stressed or transformed cells .
Retinoic acid, an active form of vitamin A, plays a significant role in the regulation of gene transcription. RAET1E is necessary for retinoid-induced differentiation in some systems . It can bind to cellular receptors structurally related to members of the nuclear receptor family, including Retinoid X receptors (RXRs), Constitutive androstane receptor (CAR), and Peroxisome proliferator-activated receptors γ/δ (PPARγ/δ) .
The recombinant form of RAET1E, produced in Sf9 insect cells, is used for various research and therapeutic purposes. The Sf9 cell line, derived from the fall armyworm (Spodoptera frugiperda), is commonly used for the expression of recombinant proteins due to its high efficiency and ability to perform post-translational modifications similar to those in mammalian cells .
