RAET1E Human, IgG-His
Retinoic Acid Early Transcript 1E, Lymphocyte Effector Toxicity Activation Ligand, RAE-1-Like Transcript 4, NKG2DL4, N2DL-4, LETAL, ULBP4, RL-4, NKG2D Ligand 4, BA350J20.7, RAET1E2, N2DL4.
Greater than 85.0% as determined by SDS-PAGE.
Description
RAET1E Human Recombinant produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 437 amino acids (31-225 a.a.) and having a molecular mass of 49.6kDa (Molecular size on SDS-PAGE will appear at approximately 57-70kDa).
RAET1E is expressed with a 239 amino acids IgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Retinoic Acid Early Transcript 1E, Lymphocyte Effector Toxicity Activation Ligand, RAE-1-Like Transcript 4, NKG2DL4, N2DL-4, LETAL, ULBP4, RL-4, NKG2D Ligand 4, BA350J20.7, RAET1E2, N2DL4.
ADPHSLCFNF TIKSLSRPGQ PWCEAQVFLN KNLFLQYNSD NNMVKPLGLL GKKVYATSTW GELTQTLGEV GRDLRMLLCD IKPQIKTSDP STLQVEMFCQ REAERCTGAS WQFATNGEKS LLFDAMNMTW TVINHEASKI KETWKKDRGL EKYFRKLSKG DCDHWLREFL GHWEAMPEPT VSPVNASDIH WSSSSLPDVE PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG KHHHHHH.
Product Science Overview
Retinoic Acid Early Transcript 1E (RAET1E) is a protein encoded by the RAET1E gene, which is located on chromosome 6q24.2-q25.3 in humans . This gene belongs to the RAET1 family, which consists of major histocompatibility complex (MHC) class I-related genes. RAET1E is a cell surface glycoprotein that plays a crucial role in the immune system by functioning as a ligand for the NKG2D receptor .
RAET1E is composed of an external α1α2 domain, a transmembrane segment, and a C-terminal cytoplasmic tail . Unlike other RAET1 proteins, RAET1E has type I membrane-spanning sequences at its C-terminus rather than glycosylphosphatidylinositol anchor sequences . This protein binds and activates the NKG2D receptor, which is expressed on the surface of several types of immune cells, including natural killer (NK) cells and certain T cells . The interaction between RAET1E and NKG2D receptor mediates natural killer cell cytotoxicity and plays a significant role in both innate and adaptive immune responses .
The human recombinant RAET1E protein with an IgG-His tag is typically produced using recombinant DNA technology. The gene encoding RAET1E is cloned into an expression vector that includes sequences for the IgG-His tag. This vector is then introduced into a suitable host cell, such as E. coli or mammalian cells, where the protein is expressed. After expression, the protein is purified using affinity chromatography techniques that exploit the His tag for efficient isolation .
RAET1E has several applications in biomedical research and therapeutic development. It is used in studies investigating the mechanisms of immune responses, particularly those involving NK cells and T cells. Additionally, RAET1E is explored as a potential target for cancer immunotherapy, as its interaction with the NKG2D receptor can enhance the cytotoxic activity of immune cells against tumor cells .
