RAET1E Human

Retinoic Acid Early Transcript 1E Human Recombinant
Cat. No.
BT14596
Source
Escherichia Coli.
Synonyms
bA350J20.7, LETAL, N2DL-4, NKG2DL4, RAET1E2, RL-4, ULBP4, NKG2D ligand 4, Retinoic acid early transcript 1E, RAE-1-like transcript 4, Lymphocyte effector toxicity activation ligand, UNQ1867/PRO4303.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

RAET1E Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 219 amino acids (31-225 a.a.) and having a molecular mass of 24.9kDa.
RAET1E is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
RAET1E, a member of the MHC class I family located on chromosome 6q24.2-q25.3, plays a crucial role in immune responses. Unlike other RAET1 proteins with glycosylphosphatidylinositol anchors, RAET1E and RAET1G possess type I membrane-spanning sequences at their C-termini. As a ligand for the NKG2D receptor found on various immune cells, RAET1E participates in both innate and adaptive immunity. It activates NK cells and promotes tumor immune surveillance by stimulating the proliferation of cytotoxic lymphocytes that target tumors.
Description
Recombinant human RAET1E, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 219 amino acids (residues 31-225). With a molecular weight of 24.9 kDa, it includes a 24 amino acid His-tag fused at the N-terminus. The protein is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
The RAET1E protein solution is provided at a concentration of 1 mg/ml in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.4 M urea, and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), keep at 4°C. For longer periods, store frozen at -20°C. To enhance stability during long-term storage, adding a carrier protein (0.1% HSA or BSA) is recommended. Avoid repeated freeze-thaw cycles.
Purity
The purity is determined to be greater than 90.0% using SDS-PAGE analysis.
Synonyms
bA350J20.7, LETAL, N2DL-4, NKG2DL4, RAET1E2, RL-4, ULBP4, NKG2D ligand 4, Retinoic acid early transcript 1E, RAE-1-like transcript 4, Lymphocyte effector toxicity activation ligand, UNQ1867/PRO4303.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMHSLCFN FTIKSLSRPG QPWCEAQVFL NKNLFLQYNS DNNMVKPLGL LGKKVYATST WGELTQTLGE VGRDLRMLLC DIKPQIKTSD PSTLQVEMFC QREAERCTGA SWQFATNGEK SLLFDAMNMT WTVINHEASK IKETWKKDRG LEKYFRKLSK GDCDHWLREF LGHWEAMPEP TVSPVNASDI HWSSSSLPD.

Product Science Overview

Structure and Function

RAET1E is composed of an external α1α2 domain, a transmembrane segment, and a C-terminal cytoplasmic tail . It functions as a stress-induced ligand for the NKG2D receptor, which is expressed on the surface of several types of immune cells, including natural killer (NK) cells and some T cells . The binding of RAET1E to the NKG2D receptor activates these immune cells, mediating cytotoxicity and contributing to the immune response .

Biological Role

RAET1E plays a crucial role in the regulation of immune responses. It is involved in both innate and adaptive immunity by acting as a ligand for the NKG2D receptor . This interaction is essential for the activation of NK cells and T cells, which are critical for the body’s defense against infections and tumors . The expression of RAET1E is induced under stress conditions, such as infection or cellular transformation, making it a key player in the immune surveillance system .

Clinical Significance

The RAET1E gene has been associated with various diseases, including familial temporal lobe epilepsy . Its role in immune regulation makes it a potential target for therapeutic interventions in conditions where the immune system is compromised or overactive. Additionally, the recombinant form of RAET1E can be used in research to study its function and interactions with other molecules in the immune system .

Research and Applications

Research on RAET1E has provided insights into its function and potential applications in immunotherapy. The recombinant form of RAET1E is used in various experimental setups to understand its role in immune cell activation and its potential as a therapeutic target . Studies have shown that manipulating the expression of RAET1E or blocking its interaction with the NKG2D receptor can modulate immune responses, offering potential strategies for treating autoimmune diseases and cancer .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

RAET1E Human, Sf9
Retinoic Acid Early Transcript 1E Human Recombinant, Sf9
Cat. No.
BT14461
Source
Sf9, Baculovirus cells.
RAET1E Mouse
Retinoic Acid Early Transcript 1E Mouse Recombinant
Cat. No.
BT14683
Source
Sf9, Insect cells.
RAET1G Human
Retinoic Acid Early Transcript 1G Human Recombinant
Cat. No.
BT14752
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.