RAET1G Human

Retinoic Acid Early Transcript 1G Human Recombinant
Cat. No.
BT14752
Source
Escherichia Coli.
Synonyms
Retinoic acid early transcript 1G protein, RAET1G, ULBP5.
Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

RAET1G Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (corresponding to Isoform 2, Uniprot accession #Q6H3X3-2) containing 198 amino acids including a 10 a.a N-terminal His tag. The total molecular mass is 22.43kDa (calculated).

Product Specs

Introduction
Belonging to the MHC class I family, Retinoic Acid Early Transcript 1G (RAET1G/ULBP5) acts as a ligand for the NKG2D receptor found on NK cell surfaces. This interaction with NKG2D is crucial for regulating anti-tumor and anti-viral immune responses mediated by NK cells. ULBP ligands are typically produced by cells that are either virally infected or cancerous. RAET1G is known to be highly expressed in the colon and various tumor cell lines, and exists in two forms: a membrane-bound isoform and a soluble isoform.
Description
Recombinant human RAET1G, produced in E. coli, is a single, non-glycosylated polypeptide chain. This chain corresponds to Isoform 2 (Uniprot accession #Q6H3X3-2) and consists of 198 amino acids, including a 10 amino acid N-terminal His tag. The calculated total molecular mass is 22.43kDa.
Physical Appearance
Lyophilized powder with a white color after filtration.
Formulation
The RAET1G protein undergoes a 0.4 µm filtration process and is subsequently lyophilized from a 0.5mg/ml solution in 20mM Tris buffer with 50mM NaCl, at a pH of 7.5.
Solubility
To prepare a working stock solution, add deionized water to the lyophilized pellet, aiming for a concentration of approximately 0.5mg/ml. Allow sufficient time for complete dissolution. It's crucial to note that RAET1G is not sterile; therefore, before using it in cell culture, filter the product through a sterile filter of appropriate pore size.
Stability
Store the lyophilized protein at -20°C. After reconstitution, aliquot the product to minimize repeated freeze-thaw cycles. Reconstituted protein remains stable at 4°C for a limited period; stability has been observed for two weeks at 4°C.
Purity
The purity of the protein is determined using SDS-PAGE and is greater than 95.0%.
Synonyms
Retinoic acid early transcript 1G protein, RAET1G, ULBP5.
Source
Escherichia Coli.
Amino Acid Sequence
MKHHHHHHASGLADPHSLCY DITVIPKFRP GPRWCAVQGQ VDEKTFLHYD CGSKTVTPVS PLGKKLNVTT AWKAQNPVLR EVVDILTEQL LDIQLENYIP KEPLTLQARM SCEQKAEGHG SGSWQLSFDG QIFLLFDSEN RMWTTVHPGA RKMKEKWEND KDMTMSFHYI SMGDCTGWLE DFLMGMDSTL EPSAGGTV.

Product Science Overview

Gene and Protein Structure

RAET1G is located on chromosome 6 and is part of a gene cluster. The gene encodes a protein that includes C-terminal transmembrane and cytoplasmic domains. However, these domains are removed through proteolytic processing, and the protein is subsequently tethered to the plasma membrane by a glycosylphosphatidylinositol (GPI)-anchor .

Function and Mechanism

The RAET1G protein is one of several ligands for the natural killer group 2, member D (NKG2D) receptor. This receptor functions as an activating receptor in both innate and adaptive immunity. The interaction between RAET1G and NKG2D receptor mediates natural killer (NK) cell cytotoxicity, which is crucial for the immune response against tumors and virally infected cells .

RAET1G has multiple isoforms with distinct functions:

  • Isoform 1: Binds and activates the KLRK1/NKG2D receptor, mediating NK cell cytotoxicity.
  • Isoform 2: Stimulates NK cells to secrete interferon-gamma (IFNG).
  • Isoform 3: Down-regulates the expression of KLRK1 and stimulates NK cells to secrete IFNG .
Clinical Significance

RAET1G is associated with various pathways, including the metabolism of proteins and the post-translational modification of GPI-anchored proteins. It is also linked to certain diseases, such as eunuchism . The protein’s role in immune response makes it a potential target for therapeutic interventions in cancer and infectious diseases.

Research and Applications

Human recombinant RAET1G is used in research to study its function and potential therapeutic applications. Recombinant proteins are produced through genetic engineering techniques, allowing scientists to investigate the protein’s structure, function, and interactions in a controlled environment .

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