RAB5B Human
RAB5B, Member RAS Oncogene Family Human Recombinant
Cat. No.
BT9820
Source
Escherichia Coli.
Synonyms
Ras-related protein Rab-5B, RAB5B.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
RAB5B Human Recombinant fused with a 23 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 238 amino acids (1-215 a.a.) and having a molecular mass of 26.1kDa. The RAB5B is purified by proprietary chromatographic techniques.
Product Specs
Introduction
RAB5B, a member of the Rab small G protein family, plays a crucial role in endocytosis and the recycling of cell surface molecules. It interacts with RIN2 and RIN3, which potentially act as GEFs, to regulate its function. Notably, suppressing RAB5B expression has been found to disrupt the neuroprotective effects mediated by group I metabotropic glutamate receptors (mGluRs).
Description
This product consists of a single, non-glycosylated polypeptide chain of RAB5B, a human recombinant protein. Produced in E. coli, it is fused with a 23 amino acid His tag at the N-terminus. This protein encompasses 238 amino acids (1-215 a.a.) and possesses a molecular mass of 26.1 kDa. The purification process involves proprietary chromatographic techniques.
Physical Appearance
A clear, sterile solution.
Formulation
The provided solution contains RAB5B (1 mg/ml) in a buffer consisting of 20mM Tris-HCl (pH 8.0), 1mM DTT, 20% glycerol, and 0.2M NaCl.
Stability
For optimal storage, refrigerate the solution at 4°C if it will be used within 2-4 weeks. For longer-term storage, freeze the solution at -20°C. To further enhance stability during long-term storage, consider adding a carrier protein such as 0.1% HSA or BSA. It's important to avoid repeated freeze-thaw cycles.
Purity
The purity of this product exceeds 90.0%, as determined by SDS-PAGE analysis.
Synonyms
Ras-related protein Rab-5B, RAB5B.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMTSRSTA RPNGQPQASK ICQFKLVLLG ESAVGKSSLV LRFVKGQFHE YQESTIGAAF LTQSVCLDDT TVKFEIWDTA GQERYHSLAP MYYRGAQAAI VVYDITNQET FARAKTWVKE LQRQASPSIV IALAGNKADL ANKRMVEYEE AQAYADDNSL LFMETSAKTA MNVNDLFLAI AKKLPKSEPQ NLGGAAGRSR GVDLHEQSQQ NKSQCCSN.
Product Science Overview
Gene and Protein Information
Function
RAB5B is primarily involved in protein transport and is believed to play a crucial role in vesicular trafficking . It enables GDP binding activity, GTP-dependent protein binding activity, and GTPase activity . These functions are essential for the regulation of membrane trafficking, particularly in the early endosome .
Biological Processes
Cellular Localization
Associated Diseases
Pathways
Research and Clinical Relevance
Given its role in essential cellular processes and its association with significant diseases, RAB5B is a subject of ongoing research. Understanding its function and regulation can provide insights into the mechanisms of diseases like ALS and Cardiofaciocutaneous Syndrome, potentially leading to new therapeutic targets.
For more detailed information, you can refer to resources like GeneCards and The Human Protein Atlas.
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