RAB21 Human
RAB21, Member RAS Oncogene Family Human Recombinant
Cat. No.
BT7456
Source
Escherichia Coli.
Synonyms
Ras-related protein Rab-21, RAB21, KIAA0118.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
RAB21 Human Recombinant fused with a 37 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 242 amino acids (18-222 a.a.) and having a molecular mass of 27.1kDa. The RAB21 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Rab21, a member of the Ras superfamily, is a small GTP-binding protein. It plays a crucial role in integrin trafficking by associating with alpha-integrin cytoplasmic domains. Rab21 acts as a positive regulator of integrin traffic, and its function is dependent on its GTP/GDP cycle and accurate localization to the membrane.
Description
This product is a recombinant human RAB21 protein with a 37 amino acid His tag at the N-terminus. It is produced in E. coli and consists of a single, non-glycosylated polypeptide chain of 242 amino acids (18-222 a.a.), with a molecular weight of 27.1 kDa. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
This solution contains RAB21 (0.5 mg/ml) in a buffer consisting of 20mM Tris-HCl (pH 8.0), 10% glycerol, 2mM DTT, 200mM NaCl, and 1mM PMSF.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freezing and thawing.
Purity
Purity is greater than 90.0% as assessed by SDS-PAGE.
Synonyms
Ras-related protein Rab-21, RAB21, KIAA0118.
Source
Escherichia Coli.
Amino Acid Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMYSF KVVLLGEGCV GKTSLVLRYC ENKFNDKHIT TLQASFLTKK LNIGGKRVNL AIWDTAGQER FHALGPIYYR DSNGAILVYD ITDEDSFQKV KNWVKELRKM LGNEICLCIV GNKIDLEKER HVSIQEAESY AESVGAKHYH TSAKQNKGIE ELFLDLCKRM IETAQVDERA KGNGSSQPGT ARRGVQIIDD EPQAQTSGGG CC.
Product Science Overview
Gene and Protein Information
Function and Mechanism
RAB21 is involved in several cellular processes, including:
- Membrane Trafficking: RAB21 regulates the endosomal trafficking of integrins, which is necessary for the successful completion of cytokinesis during mitosis in adherent cells .
- Cell Adhesion and Migration: By controlling integrin internalization and recycling, RAB21 influences cell adhesion and migration .
- Autophagy: RAB21 is activated in response to starvation-induced autophagy and regulates the SNARE protein VAMP8, which is required for autophagosome-lysosome fusion .
- Neurite Growth: RAB21 is also involved in neurite growth, contributing to the development and maintenance of the nervous system .
Clinical Significance
The expression of RAB21 is associated with various diseases and conditions:
- Glioma: High expression of RAB21 is linked to a poor prognosis in glioma patients. The tumor suppressor miR-200b downregulates RAB21, and miR-200b itself is downregulated in glioma tissues .
- Xanthinuria and Cardiofaciocutaneous Syndrome: Diseases associated with RAB21 include Xanthinuria and Cardiofaciocutaneous Syndrome .
Expression and Localization
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