RAB3D Human
Ras-related protein Rab-3D, RAB3D, GOV, RAB16, D2-2, RAD3D.
Description
RAB3D produced in E.Coli is a single, non-glycosylated polypeptide chain containing 239 amino acids (1-219 a.a) and having a molecular mass of 26.4kDa (Molecular weight on SDS-PAGE will appear higher). RAB3D is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Ras-related protein Rab-3D, RAB3D, GOV, RAB16, D2-2, RAD3D.
MGSSHHHHHH SSGLVPRGSH MASAGDTQAG PRDAADQNFD YMFKLLLIGN SSVGKTSFLF RYADDSFTPA FVSTVGIDFK VKTVYRHDKR IKLQIWDTAG QERYRTITTA YYRGAMGFLL MYDIANQESF AAVQDWATQI KTYSWDNAQV ILVGNKCDLE DERVVPAEDG RRLADDLGFE FFEASAKENI NVKQVFERLV DVICEKMNES LEPSSSSGSN GKGPAVGDAP APQPSSCSC
.Product Science Overview
RAB3D, a member of the RAS oncogene family, is a protein-coding gene that plays a crucial role in various cellular processes. It is part of the Rab family of small GTPases, which are involved in the regulation of intracellular membrane trafficking. The RAB3D gene is located on chromosome 19 and encodes a protein that is primarily involved in the regulation of exocytosis and protein transport .
The RAB3D gene is composed of multiple exons and introns, and its expression results in the production of a protein that consists of 219 amino acids. The protein has a molecular mass of approximately 26.9 kDa and contains a GTP-binding domain, which is essential for its function as a molecular switch in intracellular signaling pathways .
RAB3D is primarily involved in the regulation of the secretory pathway. It plays a significant role in the exocytosis of secretory vesicles, which is a process crucial for the release of various substances, including hormones, neurotransmitters, and enzymes, from cells. Additionally, RAB3D is involved in bone resorption, a process where bone tissue is broken down to release minerals into the bloodstream .
Mutations or dysregulation of the RAB3D gene have been associated with several diseases. Notably, it has been linked to neurodevelopmental disorders characterized by brain abnormalities, poor growth, and dysmorphic facial features. Additionally, RAB3D has been implicated in beta-thalassemia major, a blood disorder that reduces the production of hemoglobin .
Human recombinant RAB3D protein is used in various research applications to study its function and role in disease. Recombinant proteins are produced through genetic engineering techniques, where the RAB3D gene is inserted into a host organism, such as E. coli, to produce the protein in large quantities. This recombinant protein can then be purified and used in biochemical assays, structural studies, and therapeutic research .
