RAB3A Human

RAB3A, Member RAS Oncogene Family Human Recombinant
Cat. No.
BT9224
Source
Escherichia Coli.
Synonyms
Ras-related protein Rab-3A, RAB3A.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

RAB3A produced in E.Coli is a single, non-glycosylated polypeptide chain containing 240 amino acids (1-220 a.a) and having a molecular mass of 27.1kDa.
RAB3A is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Rab3A, a member of the Rab family within the small GTPase superfamily, is primarily found in neuroendocrine cells. This protein plays a crucial role in regulating exocytosis by associating with secretory vesicle membranes. It is believed to be involved in neurotransmitter release by influencing membrane flow within nerve terminals.
Description
Produced in E. coli, this non-glycosylated RAB3A protein consists of a single polypeptide chain with 240 amino acids (specifically, amino acids 1 to 220) and has a molecular weight of 27.1 kDa. For purification, a 20 amino acid His-tag is fused to the N-terminus, and proprietary chromatographic techniques are employed.
Physical Appearance
A clear solution that has been sterilized through filtration.
Formulation
This solution contains 0.5mg/ml of RAB3A protein in a buffer consisting of 20mM Tris-HCl (pH 8.0), 20% glycerol, 0.1M NaCl, and 1mM DTT.
Stability
For optimal storage: refrigerate at 4°C if the entire vial will be used within 2-4 weeks. For longer storage, freeze at -20°C. Adding a carrier protein like 0.1% HSA or BSA is recommended for extended storage. To maintain product integrity, avoid repeated freezing and thawing.
Purity
SDS-PAGE analysis indicates a purity exceeding 90.0%.
Synonyms
Ras-related protein Rab-3A, RAB3A.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MASATDSRYG QKESSDQNFD YMFKILIIGN SSVGKTSFLF RYADDSFTPA FVSTVGIDFK VKTIYRNDKR IKLQIWDTAG QERYRTITTA YYRGAMGFIL MYDITNEESF NAVQDWSTQI KTYSWDNAQV LLVGNKCDME DERVVSSERG RQLADHLGFE FFEASAKDNI NVKQTFERLV DVICEKMSES LDTADPAVTG AKQGPQLSDQ QVPPHQDCAC.

Product Science Overview

Introduction

RAB3A is a member of the RAS oncogene family, which is known for its role in various cellular processes, including cell growth, differentiation, and survival. RAB3A, specifically, is a small GTP-binding protein that plays a crucial role in regulated exocytosis and secretion.

Gene and Protein Information

The RAB3A gene is a protein-coding gene located on chromosome 19. It encodes a protein that is involved in the neurotransmitter release cycle and the innate immune system . The protein consists of 240 amino acids and has a molecular mass of approximately 27.1 kDa . It is produced in E. coli as a single, non-glycosylated polypeptide chain and is purified using proprietary chromatographic techniques .

Function

RAB3A is primarily involved in the regulation of exocytosis and secretion. It controls the recruitment, tethering, and docking of secretory vesicles to the plasma membrane . Upon stimulation, RAB3A switches to its active GTP-bound form, cycles to vesicles, and recruits effectors such as RIMS1, RIMS2, Rabphilin-3A, RPH3AL, or SYTL4 to aid in the docking of vesicles onto the plasma membrane . This process is essential for the release of neurotransmitters and other secretory products.

Biological Processes

RAB3A is involved in several biological processes, including:

  • Neurotransmitter Release: It plays a role in the regulation of dopamine release by interacting with synaptotagmin I .
  • Insulin Secretion: It stimulates insulin secretion through interaction with RIMS2 or RPH3AL effectors in pancreatic beta cells .
  • Lysosome Exocytosis and Plasma Membrane Repair: It regulates calcium-dependent lysosome exocytosis and plasma membrane repair via interaction with SYTL4 and myosin-9 .
  • Acrosome Content Secretion: It acts as a positive regulator of acrosome content secretion in sperm cells by interacting with RIMS1 .
Associated Diseases

Mutations or dysregulation of the RAB3A gene have been associated with several diseases, including:

  • Combined Oxidative Phosphorylation Deficiency 31: A disorder characterized by a deficiency in the oxidative phosphorylation pathway .
  • Cone-Rod Dystrophy, X-Linked, 3: A genetic disorder affecting the retina, leading to vision loss .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

RAB27A Human
RAB27A, Member RAS Oncogene Family Human Recombinant
Cat. No.
BT7773
Source
Escherichia Coli.
RAB5A Human
RAB5A Human Recombinant
Cat. No.
BT9736
Source
Escherichia Coli.
RAB6B Human
RAB6B, Member RAS Oncogene Family Human Recombinant
Cat. No.
BT10082
Source
Escherichia Coli.
RAB7A Human
RAB7A, Member RAS Oncogene Family Human Recombinant
Cat. No.
BT10171
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.