PTPRC Human

Protein Tyrosine Phosphatase Receptor Type C Human Recombinant
Cat. No.
BT26171
Source
Escherichia Coli.
Synonyms
LCA, LY5, B220, CD45, T200, GP180, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, PTPRC.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

PTPRC Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 257 amino acids (1031-1251a.a) and having a molecular mass of 29.6kDa.
PTPRC is fused to a 36 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Protein Tyrosine Phosphatase Receptor Type C, also known as PTPRC, plays a crucial role in regulating T- and B-cell antigen receptor signaling. As a member of the protein tyrosine phosphatase (PTP) family, it participates in various cellular processes such as cell growth, differentiation, mitosis, and oncogenic transformation. PTPRC's structure, featuring an extracellular domain, a single transmembrane segment, and two tandem intracytoplasmic catalytic domains, classifies it as a receptor type PTP.
Description
Recombinant human PTPRC, produced in E. coli, is a non-glycosylated polypeptide chain consisting of 257 amino acids (1031-1251a.a). With a molecular weight of 29.6kDa, it features a 36 amino acid His-tag fused at the N-terminus. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The PTPRC solution is provided at a concentration of 0.25mg/ml in a buffer containing 20mM Tris-HCl (pH 8.0), 0.15M NaCl, 10% glycerol, and 1mM DTT.
Stability
For optimal storage, refrigerate at 4°C if the entire vial will be used within 2-4 weeks. For extended periods, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. It is important to avoid repeated freezing and thawing of the product.
Purity
Purity exceeds 95% as determined by SDS-PAGE analysis.
Synonyms
LCA, LY5, B220, CD45, T200, GP180, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, PTPRC.
Source
Escherichia Coli.
Amino Acid Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSVMIA AQGPLKETIG DFWQMIFQRK VKVIVMLTEL KHGDQEICAQ YWGEGKQTYG DIEVDLKDTD KSSTYTLRVF ELRHSKRKDS RTVYQYQYTN WSVEQLPAEP KELISMIQVV KQKLPQKNSS EGNKHHKSTP LLIHCRDGSQ QTGIFCALLN LLESAETEEV VDIFQVVKAL RKARPGMVST FEQYQFLYDV IASTYPAQNG QVKKNNHQED KIEFDNE.

Product Science Overview

Structure and Function

PTPRC is a member of the protein tyrosine phosphatase (PTP) family, which are signaling molecules that regulate various cellular processes, including cell growth, differentiation, mitosis, and oncogenic transformation . The structure of PTPRC includes:

  • An extracellular domain
  • A single transmembrane segment
  • Two tandem intracytoplasmic catalytic domains

This structure classifies PTPRC as a receptor-type PTP . It is essential for the activation and differentiation of T cells, B cells, and other immune cells by modulating signaling pathways .

Role in Immune System

PTPRC is an essential regulator of T and B cell antigen receptor-mediated activation . It functions through direct interaction with components of the antigen receptor complexes or by activating various Src family kinases required for antigen receptor signaling . Additionally, PTPRC suppresses JAK kinases, functioning as a regulator of cytokine receptor signaling .

Clinical Significance

Disruption in the equilibrium between protein tyrosine kinase and phosphatase activity, including that of CD45, can result in immunodeficiency, autoimmunity, or malignancy . Abnormal expression of CD45 has been observed in leukemia and lymphoma .

Research and Applications

Despite extensive research over the past 50 years, the physiology and biology of CD45 still require further clarification. Key areas of ongoing research include:

  • Regulation of CD45 phosphatase activity
  • Role of the extracellular domain of CD45
  • Alternative splicing of exons
  • Function of CD45 isoforms

CD45 is also used as a diagnostic tool to identify cells of hematological origin .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

CD45 Antibody
CD45, Mouse Anti-Human
Cat. No.
BT824
Source
CD45 Antibody, Biotin
CD45, Mouse Anti-Human Biotin
Cat. No.
BT938
Source
CD45 Antibody, FITC
CD45, Mouse Anti-Human FITC
Cat. No.
BT1066
Source
B220 Antibody
B220 (B cell specific isoform of CD45), Rat Anti-Mouse
Cat. No.
BT3394
Source
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.