B220 Antibody
Description
Product Specs
Product Science Overview
CD45 is a type I transmembrane protein tyrosine phosphatase that plays a crucial role in regulating antigen receptor signaling in both B and T lymphocytes. The CD45 molecule exists in multiple isoforms, generated through alternative splicing of exons 4, 5, and 6. These isoforms are differentially expressed on various subsets of lymphocytes, allowing for the identification and characterization of these cells .
In mice, B220 is a pan-B cell marker, meaning it is expressed on all B cells throughout their development. The expression of B220 is developmentally regulated, with varying levels observed at different stages of B cell maturation. This makes B220 an invaluable marker for studying B cell biology and immunology .
The Rat Anti-Mouse B220 antibody is a monoclonal antibody that specifically binds to the B220 isoform of CD45. This antibody is widely used in research to identify and isolate B cells from mouse tissues. It is commonly used in techniques such as flow cytometry, immunohistochemistry, and immunoprecipitation .
- Flow Cytometry: The Rat Anti-Mouse B220 antibody is frequently used in flow cytometry to analyze the expression of B220 on B cells. This allows researchers to quantify and sort B cells based on their developmental stage and activation status .
- Immunohistochemistry: This antibody is also used in immunohistochemistry to visualize B cells in tissue sections. By staining tissue samples with the Rat Anti-Mouse B220 antibody, researchers can study the distribution and localization of B cells within different tissues .
- Immunoprecipitation: The Rat Anti-Mouse B220 antibody can be used to isolate B220-expressing cells from a mixture of cells. This technique is useful for studying the biochemical properties and interactions of B220 in B cells .
The study of B220 and its role in B cell development and function is essential for understanding the immune system. B cells are responsible for producing antibodies, which are crucial for the adaptive immune response. By using the Rat Anti-Mouse B220 antibody, researchers can gain insights into the mechanisms of B cell activation, differentiation, and their role in immune responses .
