CD45 Antibody

CD45 is a large glycoprotein with a molecular weight ranging from 180 to 240 kDa . The variability in its molecular weight is due to the different isoforms that arise from alternative splicing of exons 4, 5, and 6, which encode the A, B, and C determinants, respectively . These isoforms are specific to the activation and maturation state of the cell as well as the cell type .

CD45 functions as a protein tyrosine phosphatase, which is critical for the activation of T and B cell antigen receptors . It dephosphorylates specific phosphotyrosine residues on the Src family kinases, Lck and Fyn, which are essential for initiating the signaling cascade that leads to T cell activation . Additionally, CD45 interacts with various ligands, including galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1, further modulating immune responses .

Mouse anti-human CD45 antibodies are monoclonal antibodies developed in mice that specifically target the human CD45 antigen . These antibodies are widely used in various scientific applications, including:

• Flow Cytometry: For the identification and quantification of different leukocyte populations .
• Immunohistochemistry (IHC): For the localization of CD45 in tissue sections .
• Western Blotting: For the detection of CD45 protein in cell lysates .
• Immunoprecipitation: For the isolation and study of CD45 protein complexes .

These antibodies are available in various formats, including conjugated with fluorophores such as FITC, PE, and APC, which facilitate their use in flow cytometry and other fluorescence-based assays .

The use of mouse anti-human CD45 antibodies has been instrumental in advancing our understanding of the immune system. They have been used to study the distribution and function of leukocytes in various diseases, including autoimmune disorders, infections, and cancers . Additionally, these antibodies have been employed in the development of diagnostic and therapeutic strategies, highlighting their significance in both basic and clinical research .