CD45 Antibody, Biotin

CD45 is a single-chain type I membrane glycoprotein. It has multiple isoforms that arise from the variable splicing of exons 4, 5, and 6, which encode the A, B, and C determinants, respectively . These isoforms are specific to the activation and maturation state of the cell as well as the cell type . The primary ligands for CD45 include galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1 .

CD45 is a signaling molecule that regulates various cellular processes, including cell growth, differentiation, cell cycle, and oncogenic transformation . It plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates such as p56Lck, p59Fyn, and other Src family kinases . CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes .

The mouse anti-human CD45 antibody is a monoclonal antibody that has been conjugated with biotin. This conjugation is achieved through affinity chromatography, ensuring optimal conditions for the biotin attachment . The biotinylated antibody is commonly used in various applications, including flow cytometry, immunohistochemistry, and Western blotting .

1. Flow Cytometry: The biotinylated mouse anti-human CD45 antibody is used for immunofluorescent staining followed by flow cytometric analysis. It is recommended to use ≤0.125 µg per million cells in a 100 µl volume .
2. Immunohistochemistry: This antibody can be used for staining acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections .
3. Western Blotting: The antibody is also suitable for Western blotting applications .

The antibody solution should be stored undiluted between 2°C and 8°C and should not be frozen . Proper storage ensures the stability and functionality of the antibody for its intended applications.