CD45 Antibody, FITC
Description
Product Specs
Product Science Overview
CD45, also known as the leukocyte common antigen (LCA), is a 180-240 kDa glycoprotein expressed on the surface of all hematopoietic cells, except mature erythrocytes and platelets . It plays a crucial role in the regulation of antigen receptor signaling in both T and B cells by acting as a protein tyrosine phosphatase . The CD45 molecule exists in multiple isoforms due to alternative splicing of exons 4, 5, and 6, which encode the A, B, and C determinants, respectively .
The mouse anti-human CD45 antibody is a monoclonal antibody that specifically binds to human CD45. This antibody is typically conjugated with fluorescein isothiocyanate (FITC), a fluorescent dye that allows for the detection and analysis of CD45 expression on cells using flow cytometry . The FITC conjugation facilitates the visualization of CD45 on the cell surface, making it a valuable tool in immunological research and clinical diagnostics .
The mouse anti-human CD45 antibody is produced by immunizing mice with human peripheral blood mononuclear cells (PBMCs) or other relevant human tissues . The resulting hybridoma cells are then screened for the production of antibodies that specifically bind to human CD45. These antibodies are purified using affinity chromatography and subsequently conjugated with FITC under optimal conditions .
The FITC-conjugated mouse anti-human CD45 antibody is widely used in various applications, including:
- Flow Cytometry: This antibody is commonly used to stain human leukocytes for flow cytometric analysis, allowing researchers to study the expression of CD45 on different cell populations .
- Immunohistochemistry: The antibody can be used to detect CD45 expression in tissue sections, providing valuable information about the distribution and density of leukocytes in different tissues .
- Western Blotting: It can also be employed in Western blotting to detect CD45 protein levels in cell lysates .
CD45 is a critical regulator of immune cell function, and its expression is essential for the proper activation and differentiation of T and B cells . The use of FITC-conjugated mouse anti-human CD45 antibodies has greatly advanced our understanding of immune cell biology and has facilitated numerous studies in immunology and clinical diagnostics .
