PSMB10 Human
Proteasome subunit beta type-10, Low molecular mass protein 10, Macropain subunit MECl-1, Multicatalytic endopeptidase complex subunit MECl-1, Proteasome MECl-1, Proteasome subunit beta-2i, PSMB10, LMP10, MECL1, beta2i, MGC1665, FLJ00366.
Description
PSMB10 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 255 amino acids (40-273 a.a.) and having a molecular mass of 26.9kDa.
PSMB10 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Proteasome subunit beta type-10, Low molecular mass protein 10, Macropain subunit MECl-1, Multicatalytic endopeptidase complex subunit MECl-1, Proteasome MECl-1, Proteasome subunit beta-2i, PSMB10, LMP10, MECL1, beta2i, MGC1665, FLJ00366.
MGSSHHHHHH SSGLVPRGSH MTTIAGLVFQ DGVILGADTR ATNDSVVADK SCEKIHFIAP KIYCCGAGVA ADAEMTTRMV ASKMELHALS TGREPRVATV TRILRQTLFR YQGHVGASLI VGGVDLTGPQ LYGVHPHGSY SRLPFTALGS GQDAALAVLE DRFQPNMTLE AAQGLLVEAV
TAGILGDLGS GGNVDACVIT KTGAKLLRTL SSPTEPVKRS GRYHFVPGTT AVLTQTVKPL TLELVEETVQ AMEVE.
Product Science Overview
Proteasome Beta Type 10 (PSMB10) is a subunit of the proteasome complex, specifically a part of the 20S core particle. The proteasome is a multicatalytic proteinase complex that plays a crucial role in degrading unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds. This process is essential for maintaining cellular homeostasis and regulating various cellular processes.
The 20S core of the proteasome is composed of four stacked rings, forming a barrel-like structure. Each ring consists of seven subunits: the two outer rings are made up of alpha subunits, while the two inner rings are composed of beta subunits. PSMB10 is one of these beta subunits and is also known as the low molecular mass polypeptide 10 (LMP10) or multicatalytic endopeptidase complex-like 1 (MECL1) .
PSMB10 is involved in the proteolytic processing required to generate a mature subunit. It is induced by gamma interferon and replaces the catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome. The immunoproteasome is a modified form of the proteasome that plays a key role in the immune system by processing class I MHC peptides .
The expression of PSMB10 is regulated by various factors, including gamma interferon. This regulation is crucial for the formation of the immunoproteasome, which is involved in the immune response. The immunoproteasome enhances the generation of antigenic peptides that are presented by MHC class I molecules to cytotoxic T cells, thus playing a vital role in the immune surveillance against pathogens and tumors .
Mutations or dysregulation of PSMB10 can be associated with various diseases. For instance, it has been linked to proteasome-associated autoinflammatory syndrome 5 (PRAAS5), a rare genetic disorder characterized by chronic inflammation. Additionally, alterations in proteasome function, including that of PSMB10, have been implicated in neurodegenerative diseases, cancer, and other conditions where protein homeostasis is disrupted .
Recombinant PSMB10 is produced using various expression systems, such as Escherichia coli, to study its structure and function in detail. The recombinant protein is often tagged with His tags for purification purposes and is used in various biochemical assays to understand its role in the proteasome complex .
