PSMB8 Human
Proteasome Subunit Beta Type 8 Human Recombinant
Cat. No.
BT6951
Source
Escherichia Coli.
Synonyms
D6S216, D6S216E, LMP7, MGC1491, PSMB5i , Low molecular mass protein 7 Macropain subunit C13, Multicatalytic endopeptidase complex subunit C13,Proteasome component C13, Proteasome subunit beta-5i, Really interesting new gene 10 protein.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
PSMB8 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 229 amino acids (73-276 a.a.) and having a molecular mass of 25.4kDa. PSMB8 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Proteasome Subunit Beta Type 8 (PSMB8), also known as LMP7, is a component of the immunoproteasome, a specialized form of the proteasome primarily found in immune cells. The proteasome is a large protein complex responsible for degrading intracellular proteins targeted for destruction. PSMB8 is involved in the processing of antigens for presentation on major histocompatibility complex (MHC) class I molecules, which is crucial for immune recognition and response. PSMB8 is primarily expressed in immature dendritic cells and plays a role in INF-gamma-induced sensitivity and apoptosis by degrading the apoptotic inhibitor MCL1.
Description
Recombinant human PSMB8 protein was expressed in E. coli and purified to yield a single, non-glycosylated polypeptide chain. The protein contains 229 amino acids (residues 73-276) and has a molecular weight of 25.4 kDa. For purification and detection purposes, a 25 amino acid His-tag is present at the N-terminus.
Physical Appearance
Clear, colorless and sterile-filtered solution.
Formulation
The PSMB8 protein is supplied in a solution at a concentration of 0.5 mg/ml. The buffer consists of 20mM Tris-HCl (pH 8.0), 0.4M Urea, and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), the PSMB8 protein solution should be stored at 4°C. For long-term storage, it is recommended to store the protein at -20°C. Adding a carrier protein such as HSA or BSA to a final concentration of 0.1% is advised for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of the PSMB8 protein is greater than 85%, as determined by SDS-PAGE analysis.
Synonyms
D6S216, D6S216E, LMP7, MGC1491, PSMB5i , Low molecular mass protein 7 Macropain subunit C13, Multicatalytic endopeptidase complex subunit C13,Proteasome component C13, Proteasome subunit beta-5i, Really interesting new gene 10 protein.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMTTTLA FKFQHGVIAA VDSRASAGSY ISALRVNKVI EINPYLLGTM SGCAADCQYW ERLLAKECRL YYLRNGERIS VSAASKLLSN MMCQYRGMGL SMGSMICGWD KKGPGLYYVD EHGTRLSGNM FSTGSGNTYA YGVMDSGYRP NLSPEEAYDL GRRAIAYATH RDSYSGGVVN MYHMKEDGWV KVESTDVSDL LHQYREANQ.
Product Science Overview
Structure and Function
The human PSMB8 protein is composed of 204 amino acids and has a molecular weight of approximately 23 kDa . It is one of the 17 essential subunits that contribute to the complete assembly of the 20S proteasome complex . The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure, composed of four rings of 28 non-identical subunits: two rings of seven alpha subunits and two rings of seven beta subunits .
The primary function of the proteasome is to degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds . The immunoproteasome, a modified form of the proteasome, plays a critical role in the processing of class I MHC peptides, which are essential for the immune response .
Biological Processes
PSMB8 is involved in several biological processes, including:
- Antigen processing and presentation: It processes exogenous peptide antigens via MHC class I, TAP-dependent pathway .
- Regulation of cellular amino acid metabolic process .
- Protein polyubiquitination: It is involved in the proteasome-mediated ubiquitin-dependent protein catabolic process .
- Immune system processes: It plays a role in the immune response, including the type I interferon signaling pathway and the tumor necrosis factor-mediated signaling pathway .
Expression and Regulation
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