PSMB3 Human

Proteasome Subunit Beta Type 3 Human Recombinant
Cat. No.
BT5933
Source
Escherichia Coli.
Synonyms
Proteasome (prosome, macropain) subunit beta type 3, Proteasome chain 13, HC10-II, Proteasome component C10-II, Proteasome theta chain, EC 3.4.25.1, MGC4147.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PSMB3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 225 amino acids (1-205 a.a.) and having a molecular mass of 25.1kDa.
PSMB3 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The proteasome, a multi-catalytic proteinase complex, possesses a highly organized ring-shaped 20S core structure. This core comprises four rings of 28 distinct subunits: two rings of seven alpha subunits and two rings of seven beta subunits. Abundantly distributed throughout eukaryotic cells, proteasomes play a crucial role in the ATP/ubiquitin-dependent degradation of peptides via a non-lysosomal pathway. Notably, a specialized form of the proteasome, the immunoproteasome, is essential for processing class I MHC peptides.
Description
Recombinantly produced in E. coli, PSMB3 Human is a single, non-glycosylated polypeptide chain comprising 225 amino acids (specifically, residues 1 to 205) and exhibiting a molecular mass of 25.1 kDa. This protein is engineered with a 20 amino acid His-tag fused to its N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The PSMB3 protein solution is provided at a concentration of 0.25 mg/ml in a buffer containing 20 mM Tris-HCl (pH 8.0), 200 mM NaCl, 1 mM DTT, and 50% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, freezing at -20°C is recommended. The addition of a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Importantly, repeated freeze-thaw cycles should be avoided.
Purity
SDS-PAGE analysis indicates a purity exceeding 90.0%.
Synonyms
Proteasome (prosome, macropain) subunit beta type 3, Proteasome chain 13, HC10-II, Proteasome component C10-II, Proteasome theta chain, EC 3.4.25.1, MGC4147.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MSIMSYNGGA VMAMKGKNCV AIAADRRFGI QAQMVTTDFQ KIFPMGDRLY IGLAGLATDV QTVAQRLKFR LNLYELKEGR QIKPYTLMSM VANLLYEKRF GPYYTEPVIA GLDPKTFKPF ICSLDLIGCP MVTDDFVVSG TCAEQMYGMC ESLWEPNMDP DHLFETISQA MLNAVDRDAV SGMGVIVHII EKDKITTRTL KARMD

Product Science Overview

Structure and Function

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. This core structure is composed of four rings of 28 non-identical subunits: two rings are composed of seven alpha subunits, and two rings are composed of seven beta subunits . PSMB3 is one of these beta subunits and is part of the proteasome B-type family, also known as the T1B family .

The 20S proteasome complex is involved in the ATP-dependent degradation of ubiquitinated proteins. This process is essential for maintaining cellular homeostasis by regulating the concentration of specific proteins and degrading misfolded proteins . The proteasome also plays a role in various cellular processes, including the regulation of the cell cycle, modulation of various signaling pathways, and antigen processing for immune responses .

Clinical Significance

Mutations or dysregulation of the PSMB3 gene have been associated with several diseases, including Cystic Fibrosis and Parkinson’s Disease . The proteasome’s role in degrading misfolded proteins is particularly relevant in neurodegenerative diseases, where the accumulation of misfolded proteins can lead to cellular dysfunction and disease progression .

Research and Applications

Recombinant PSMB3 is used in various research applications to study its structure, function, and role in disease. Understanding the mechanisms by which PSMB3 and the proteasome complex operate can provide insights into potential therapeutic targets for diseases associated with proteasome dysfunction .

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