PSMB5 Human
Description
Product Specs
Product Science Overview
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. This core structure is composed of four rings of 28 non-identical subunits: two rings are composed of seven alpha subunits, and two rings are composed of seven beta subunits . PSMB5 is one of the beta subunits and is essential for the proteasome’s proteolytic activity .
The primary function of the proteasome is to degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds . This process is crucial for maintaining the cell’s health and function by regulating the concentration of specific proteins and degrading misfolded proteins .
The PSMB5 gene is located on chromosome 14 at band 14q11.2 . It consists of five exons and encodes a member of the proteasome B-type family, also known as the T1B family . The gene is expressed in various tissues, with high expression levels in the gastrocnemius muscle, islet of Langerhans, stromal cells of the endometrium, and several other tissues .
PSMB5 is involved in several biological processes, including:
- Regulation of cellular amino acid metabolic process
- Antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
- Regulation of mRNA stability
- Positive regulation of canonical Wnt signaling pathway
- Protein polyubiquitination
- Response to oxidative stress
- Proteolysis involved in cellular protein catabolic process
- T cell receptor signaling pathway
- Negative regulation of canonical Wnt signaling pathway
- Proteasome-mediated ubiquitin-dependent protein catabolic process
- Viral process
- Regulation of transcription from RNA polymerase II promoter in response to hypoxia
- Post-translational protein modification
- Regulation of hematopoietic stem cell differentiation
- Proteasomal protein catabolic process
- Interleukin-1-mediated signaling pathway
- Regulation of mitotic cell cycle phase transition .
Mutations or dysregulation of the PSMB5 gene have been associated with various diseases, including Parkinson’s Disease and Cystic Fibrosis . The proteasome’s role in degrading misfolded proteins is particularly relevant in neurodegenerative diseases, where the accumulation of misfolded proteins is a hallmark .
Recombinant PSMB5 is produced using recombinant DNA technology, which involves inserting the PSMB5 gene into a suitable expression system, such as bacteria or yeast, to produce the protein in large quantities. This recombinant protein is used in various research applications, including studying the proteasome’s structure and function, screening for proteasome inhibitors, and understanding the role of the proteasome in different diseases.
