PSMB2 Human

Proteasome Subunit Beta Type 2 Human Recombinant
Cat. No.
BT5845
Source
Escherichia Coli.
Synonyms
Proteasome subunit beta type-2, Macropain subunit C7-I, Multicatalytic endopeptidase complex subunit C7-I, Proteasome component C7-I, PSMB2, HC7-I.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PSMB2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 221 amino acids (1-201) and having a molecular mass of 24.9kDa.
PSMB2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Proteasome subunit beta type-2 (PSMB2), a member of the proteasome T1B family, is a 20S core beta subunit. The proteasome, a multicatalytic proteinase complex, possesses a highly ordered ring-shaped 20S core structure. Widely distributed within eukaryotic cells, proteasomes play a crucial role in the ATP/ubiquitin-dependent degradation of peptides through a non-lysosomal pathway. The immunoproteasome, a modified proteasome, plays a critical role in processing class I MHC peptides. Notably, PSMB2 exhibits elevated expression levels in ovarian cancer cell lines.
Description
Recombinant human PSMB2, produced in E. coli, is a single, non-glycosylated polypeptide chain composed of 221 amino acids (residues 1-201), resulting in a molecular weight of 24.9 kDa. The protein features a 20-amino acid His-tag fused to its N-terminus and undergoes purification using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The PSMB2 solution is provided at a concentration of 0.5 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 1 mM DTT, 30% glycerol, and 0.1 M NaCl.
Stability
For short-term storage (up to 2-4 weeks), the product should be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freezing and thawing should be minimized.
Purity
Purity exceeds 90.0% as assessed by SDS-PAGE.
Synonyms
Proteasome subunit beta type-2, Macropain subunit C7-I, Multicatalytic endopeptidase complex subunit C7-I, Proteasome component C7-I, PSMB2, HC7-I.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MEYLIGIQGP DYVLVASDRV AASNIVQMKD DHDKMFKMSE KILLLCVGEA GDTVQFAEYI QKNVQLYKMR NGYELSPTAA ANFTRRNLAD CLRSRTPYHV NLLLAGYDEH EGPALYYMDY LAALAKAPFA AHGYGAFLTL SILDRYYTPT ISRERAVELL
RKCLEELQKR FILNLPTFSV RIIDKNGIHD LDNISFPKQG S.

Product Science Overview

Introduction

Proteasome Subunit Beta Type 2 (PSMB2), also known as 20S proteasome subunit beta-4, is a crucial component of the proteasome complex in humans. This protein is encoded by the PSMB2 gene and plays a significant role in the degradation of intracellular proteins, which is essential for maintaining cellular homeostasis .

Structure and Function

The human PSMB2 protein is composed of 201 amino acids and has a molecular weight of approximately 23 kDa . It is a non-catalytic component of the 20S core proteasome complex, which is involved in the proteolytic degradation of most intracellular proteins . The proteasome complex is responsible for various cellular processes, including the regulation of the cell cycle, modulation of signaling pathways, and removal of damaged or misfolded proteins .

Expression and Localization

PSMB2 is expressed in various tissues, with high expression levels observed in the mucosa of the ileum, tibialis anterior muscle, deltoid muscle, and several other tissues . The protein is localized in the cytoplasm, cytosol, membrane, nucleoplasm, and proteasome complex .

Biological Processes

PSMB2 is involved in numerous biological processes, including:

  • Regulation of cellular amino acid metabolic processes
  • Antigen processing and presentation of exogenous peptide antigens via MHC class I
  • Regulation of mRNA stability
  • Positive regulation of the canonical Wnt signaling pathway
  • Protein polyubiquitination
  • Tumor necrosis factor-mediated signaling pathway
Clinical Significance

PSMB2 has been associated with various clinical conditions. For instance, it has shown stability in bronchoalveolar cells of the lung during certain clinical conditions such as interstitial lung disease and sarcoidosis . Additionally, diseases associated with PSMB2 include acute myeloid leukemia and Schimke immunoosseous dysplasia .

Recombinant PSMB2

Recombinant human PSMB2 is often produced using Escherichia coli expression systems. The recombinant protein is typically purified to a high degree of purity (>90%) and is used in various research applications, including mass spectrometry and SDS-PAGE . The recombinant form of PSMB2 retains the functional properties of the native protein, making it a valuable tool for studying proteasome function and related cellular processes .

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