PSMA7 Human
Proteasome Subunit Alpha Type 7 Human Recombinant
Cat. No.
BT5505
Source
Escherichia Coli.
Synonyms
HSPC, RC6-1, XAPC7, MGC3755, PSMA-7, Proteasome subunit alpha type-7, Proteasome subunit RC6-1, Proteasome subunit XAPC7, PSMA7, C6.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
PSMA7 Human Recombinant fused to N-terminal His-Tag produced in E.Coli is a single, non-glycosylated polypeptide chain containing 268 amino acids (1-248) and having a molecular mass of 30kDa. PSMA7 is fused to a 20 amino acid His Tag at N-Terminus and purified by standard chromatography techniques.
Product Specs
Introduction
PSMA7, also known as Proteasome subunit alpha type-7, is a crucial component of the 20S core particle in the proteasome complex. This complex is responsible for breaking down proteins in the cell through an ATP/ubiquitin-dependent pathway. PSMA7, being an alpha subunit, contributes to the structural integrity of the proteasome. Beyond its role in protein degradation, PSMA7 is involved in the life cycle of the hepatitis B virus by interacting with the viral X protein, essential for viral replication. It also influences the activity of the hepatitis C virus internal ribosome entry site, a critical element for viral replication. Furthermore, PSMA7 plays a role in regulating cellular responses to oxygen levels by interacting with and regulating HIF-1alpha, a transcription factor that responds to changes in oxygen concentration. This interaction impacts the ability of HIF-1alpha to activate genes involved in adapting to low oxygen conditions. Enzymatically, PSMA7 exhibits a preference for cleaving peptides at specific amino acid residues (Arg, Phe, Tyr, Leu, and Glu) adjacent to the leaving group, with optimal activity at neutral to slightly basic pH.
Description
PSMA7 Human Recombinant, tagged with N-terminal His-Tag, is produced in E. coli. This protein is a single, non-glycosylated polypeptide chain containing 268 amino acids (residues 1-248), with a molecular weight of 30 kDa. The recombinant PSMA7 is purified using standard chromatography techniques following its expression in E. coli.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The PSMA7 protein is supplied in a solution containing 20mM Tris-HCl buffer at pH 8.0, 0.1M NaCl, and 20% glycerol.
Stability
For short-term storage (up to 4 weeks), the PSMA7 solution can be stored at 4°C. For extended storage, it is recommended to store the protein in aliquots at -20°C. To ensure long-term stability during frozen storage, the addition of a carrier protein such as HSA or BSA to a final concentration of 0.1% is advisable. Repeated freezing and thawing of the protein should be avoided to maintain its integrity.
Purity
The purity of PSMA7 Human Recombinant is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
HSPC, RC6-1, XAPC7, MGC3755, PSMA-7, Proteasome subunit alpha type-7, Proteasome subunit RC6-1, Proteasome subunit XAPC7, PSMA7, C6.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MSYDRAITVF SPDGHLFQVE YAQEAVKKGS TAVGVRGRDI VVLGVEKKSV AKLQDERTVR KICALDDNVC MAFAGLTADA RIVINRARVE CQSHRLTVED PVTVEYITRY IASLKQRYTQ SNGRRPFGIS ALIVGFDFDG TPRLYQTDPS GTYHAWKANA IGRGAKSVRE FLEKNYTDEA IETDDLTIKL VIKALLEVVQ SGGKNIELAV MRRDQSLKIL NPEEIEKYVA EIEKEKEENE KKKQKKAS.
Product Science Overview
Structure and Function
PSMA7 is part of the alpha ring of the proteasome, contributing to the formation of heptameric alpha rings and the substrate entrance gate . The 20S proteasome complex, in association with different regulatory particles, forms the 26S proteasome. This complex is essential for the ATP-dependent degradation of ubiquitinated proteins, which is crucial for maintaining protein homeostasis by removing misfolded or damaged proteins .
Biological Roles
PSMA7 is involved in various cellular processes, including:
- Protein Degradation: It mediates the degradation of intracellular proteins, both ubiquitin-dependent and ubiquitin-independent .
- Regulation of Transcription Factors: It inhibits the transactivation function of HIF-1A under both normoxic and hypoxic conditions .
- Immune Response: It plays a role in the generation of MHC class I-presented antigenic peptides .
- Cancer Progression: PSMA7 has been shown to promote proliferation and metastasis in gastric cancer through the MAPK signaling pathway .
Clinical Significance
Research and Applications
Recombinant PSMA7 is used in research to study its structure, function, and role in various diseases. Understanding its mechanisms can lead to the development of new therapeutic strategies for diseases associated with proteasome dysfunction.
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