PSMA4 Human
Description
PSMA4 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
The 20S proteasome is a highly ordered, ring-shaped structure composed of four stacked rings, each containing seven subunits . The two outer rings consist of alpha subunits, including PSMA4, while the two inner rings are made up of beta subunits . This arrangement forms a barrel-like structure with a central cavity where protein degradation occurs .
PSMA4, as part of the 20S core proteasome, is involved in the ATP- and ubiquitin-dependent degradation of proteins . This process is essential for maintaining cellular homeostasis by removing misfolded or damaged proteins that could impair cellular functions . Additionally, the proteasome regulates various cellular processes, including the cell cycle, apoptosis, and DNA repair .
The 20S proteasome can associate with different regulatory particles to form larger complexes, such as the 26S proteasome . The 26S proteasome, which includes two 19S regulatory particles, is responsible for the ATP-dependent degradation of ubiquitinated proteins . This complex plays a key role in maintaining protein homeostasis and regulating various cellular pathways .
In addition to its role in ubiquitin-dependent degradation, the 20S proteasome can also mediate ubiquitin-independent protein degradation when associated with regulatory particles like PA200 or PA28 . This type of proteolysis is required in several pathways, including spermatogenesis and the generation of a subset of MHC class I-presented antigenic peptides .
Recombinant PSMA4 is a human full-length protein expressed in Escherichia coli, with a purity greater than 95% . It is suitable for various applications, including SDS-PAGE and mass spectrometry (MS) . The availability of recombinant PSMA4 allows researchers to study its structure, function, and interactions in detail, contributing to a better understanding of its role in cellular processes and potential implications in diseases.
Mutations or dysregulation of the PSMA4 gene have been associated with several diseases, including cystic fibrosis and tobacco addiction . Understanding the function and regulation of PSMA4 and the proteasome complex can provide insights into the mechanisms underlying these conditions and potentially lead to the development of targeted therapies.
