PSMA2 Human
Proteasome subunit alpha type-2, Macropain subunit C3, Multicatalytic endopeptidase complex subunit C3, Proteasome component C3, PSMA2, HC3, PSC3, MU, PSC2.
Description
PSMA2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 254 amino acids (1-234 a.a.) and having a molecular mass of 28kDa.
PSMA2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Proteasome subunit alpha type-2, Macropain subunit C3, Multicatalytic endopeptidase complex subunit C3, Proteasome component C3, PSMA2, HC3, PSC3, MU, PSC2.
MGSSHHHHHH SSGLVPRGSH MAERGYSFSL TTFSPSGKLV QIEYALAAVA GGAPSVGIKA ANGVVLATEK KQKSILYDER SVHKVEPITK HIGLVYSGMG PDYRVLVHRA RKLAQQYYLV YQEPIPTAQL VQRVASVMQE YTQSGGVRPF GVSLLICGWN EGRPYLFQSD PSGAYFAWKA TAMGKNYVNG KTFLEKRYNE DLELEDAIHT AILTLKESFE GQMTEDNIEV GICNEAGFRR LTPTEVKDYL AAIA.
Product Science Overview
Proteasome Subunit Alpha Type 2, also known as PSMA2, is a crucial component of the proteasome complex in humans. This protein is encoded by the PSMA2 gene and plays a significant role in the degradation of intracellular proteins. The recombinant form of this protein is often used in research to study its structure and function.
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. This core is composed of four rings of 28 non-identical subunits: two rings of seven alpha subunits and two rings of seven beta subunits . PSMA2 is one of the alpha subunits and is part of the 20S core proteasome complex .
The primary function of the proteasome is to degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds. This process is ATP-dependent and involves the tagging of target proteins with ubiquitin, marking them for degradation . The 20S core proteasome can associate with different regulatory particles, such as the 19S regulatory particle, to form the 26S proteasome, which is involved in the ATP-dependent degradation of ubiquitinated proteins .
Proteasomes are distributed throughout eukaryotic cells at high concentrations and are essential for maintaining cellular homeostasis. They play a critical role in various cellular processes, including the regulation of the cell cycle, modulation of various signaling pathways, and the removal of misfolded or damaged proteins . The immunoproteasome, a modified form of the proteasome, is involved in the processing of class I MHC peptides, which are crucial for the immune response .
Recombinant human PSMA2 is produced using recombinant DNA technology, typically in bacterial expression systems such as Escherichia coli. The recombinant protein often includes a His-tag at the N-terminus to facilitate purification . This recombinant form is used in various research applications, including structural studies, functional assays, and drug discovery .
Recombinant PSMA2 is valuable in research for several reasons:
- Structural Studies: Understanding the structure of PSMA2 helps in elucidating the overall architecture of the proteasome complex.
- Functional Assays: Researchers use recombinant PSMA2 to study its enzymatic activity and its role in protein degradation.
- Drug Discovery: PSMA2 is a target for drug discovery efforts aimed at modulating proteasome activity, which has implications in treating diseases such as cancer and neurodegenerative disorders .
