PSMA6 Human

Proteasome Subunit Alpha Type 6 Human Recombinant
Cat. No.
BT5411
Source
Escherichia Coli.
Synonyms
Proteasome (prosome, macropain) subunit alpha type 6, PROS27, p27K, IOTA, Macropain iota chain, Multicatalytic endopeptidase complex iota chain, Proteasome iota chain, 27 kDa prosomal protein.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PSMA6 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 270 amino acids (1-246 a.a.) and having a molecular mass of 29.9kDa.
PSMA6 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
PSMA6, a member of the peptidase T1A family, is a 20S core alpha subunit. The proteasome, a multicatalytic proteinase complex, possesses a highly organized ring-shaped 20S core structure composed of four rings of 28 distinct subunits. These rings consist of two rings of seven alpha subunits and two rings of seven beta subunits. PSMA6 is widely distributed throughout eukaryotic cells and plays a crucial role in the ATP/ubiquitin-dependent degradation of peptides within a non-lysosomal pathway.
Description
Recombinant human PSMA6, expressed in E. coli, is a single, non-glycosylated polypeptide chain with a molecular weight of 29.9 kDa. It comprises 270 amino acids, including a 24 amino acid His-tag fused to the N-terminus. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The PSMA6 protein solution is provided at a concentration of 0.25 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 1 mM DTT, and 40% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product should be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure long-term stability, adding a carrier protein such as HSA or BSA (0.1%) is advised. It is crucial to avoid repeated cycles of freezing and thawing.
Purity
The purity of the PSMA6 protein is greater than 90.0% as determined by SDS-PAGE analysis.
Synonyms
Proteasome (prosome, macropain) subunit alpha type 6, PROS27, p27K, IOTA, Macropain iota chain, Multicatalytic endopeptidase complex iota chain, Proteasome iota chain, 27 kDa prosomal protein.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMSRGSS AGFDRHITIF SPEGRLYQVE YAFKAINQGG LTSVAVRGKD CAVIVTQKKV PDKLLDSSTVTHLFKITENI GCVMTGMTAD SRSQVQRARY EAANWKYKYG YEIPVDMLCK RIADISQVYT QNAEMRPLGC CMILIGIDEE QGPQVYKCDP AGYYCGFKAT AAGVKQTEST SFLEKKVKKK FDWTFEQTVE TAITCLSTVL SIDFKPSEIE VGVVTVENPK FRILTEAEID AHLVALAERD

Product Science Overview

Structure and Composition

PSMA6 is a protein composed of 246 amino acids with a molecular weight of approximately 27 kDa . The theoretical isoelectric point (pI) of this protein is 6.35 . It is expressed in various tissues and is a part of the 20S core proteasome complex, which is involved in the proteolytic degradation of most intracellular proteins .

Function

The primary function of PSMA6 is to participate in the ATP-dependent degradation of ubiquitinated proteins by forming the 26S proteasome when associated with two 19S regulatory particles . This process is essential for maintaining protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions . Additionally, PSMA6 is involved in various cellular processes, including the regulation of the cell cycle, apoptosis, and the immune response .

Clinical Significance

Mutations or dysregulation of the PSMA6 gene have been associated with several diseases, including myocardial infarction and inflammatory bowel disease . Research has also shown that PSMA6 is critical for the survival of pancreatic ductal carcinoma cell models, indicating its potential role in cancer biology .

Recombinant PSMA6

Recombinant human PSMA6 is produced using Escherichia coli expression systems and is typically purified to a high degree of purity (>90%) suitable for various applications such as SDS-PAGE and mass spectrometry (MS) . This recombinant protein is valuable for research purposes, including studying the structure and function of the proteasome complex and its role in disease mechanisms.

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