PSMA5 Human
Description
PSMA5 is fused to a 36 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Proteasome Subunit Alpha Type 5, also known as PSMA5, is a crucial component of the 20S core proteasome complex in humans. This protein is encoded by the PSMA5 gene and plays a significant role in the proteolytic degradation of intracellular proteins. The proteasome is essential for maintaining cellular homeostasis by degrading damaged or misfolded proteins and regulating various cellular processes.
The 20S proteasome core is a barrel-shaped complex composed of four stacked rings, each containing seven subunits. The two outer rings consist of alpha subunits, while the two inner rings are made up of beta subunits. PSMA5 is one of the seven alpha subunits that form the outer rings of the 20S proteasome .
The PSMA5 protein is 26.5 kDa in size and consists of 241 amino acids. It is a member of the peptidase T1A family and has a calculated theoretical isoelectric point (pI) of 4.69 . The gene encoding PSMA5 is located on chromosome 1p13 and contains nine exons .
The primary function of the proteasome is to degrade ubiquitinated proteins in an ATP-dependent manner. The 20S core proteasome, in association with regulatory particles, forms the 26S proteasome, which is responsible for the ATP-dependent degradation of ubiquitinated proteins . The proteasome plays a vital role in various cellular processes, including cell cycle regulation, apoptosis, and the immune response.
PSMA5, as part of the alpha ring, contributes to the formation of the heptameric alpha rings and the substrate entrance gate. The alpha subunits regulate the entry of substrates into the proteolytic chamber formed by the beta subunits .
Mutations or dysregulation of the PSMA5 gene can lead to various diseases. For example, PSMA5 has been associated with conditions such as Freemartinism and Cystic Fibrosis . Additionally, the proteasome is a target for cancer therapy, as its inhibition can lead to the accumulation of damaged proteins and induce apoptosis in cancer cells.
Recombinant PSMA5 is used in research to study the structure and function of the proteasome. It is also utilized in drug discovery and development, particularly in the design of proteasome inhibitors for cancer therapy. Understanding the role of PSMA5 in the proteasome complex can provide insights into the mechanisms of protein degradation and the development of therapeutic strategies for various diseases.
