PSMA3 Human

Proteasome Subunit Alpha Type 3 Human Recombinant
Cat. No.
BT5214
Source
E.coli.
Synonyms
proteasome (prosome, macropain) subunit alpha type-3, HC8, Macropain subunit C8, Multicatalytic endopeptidase complex subunit C8, Proteasome component C8, PSC3, PSC8, EC 3.4.25.1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PSMA3 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 275 amino acids (1-255) and having a molecular mass of 30.6 kDa.
PSMA3 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The proteasome, a vital multicatalytic proteinase complex, possesses a highly organized 20S core structure resembling a ring. This core comprises four rings of 28 distinct subunits: two rings consisting of 7 alpha subunits and two rings consisting of 7 beta subunits. Proteasomes are abundant throughout eukaryotic cells, playing a critical role in the ATP/ubiquitin-dependent degradation of peptides through a non-lysosomal pathway. The PSMA3 gene encodes several members of the peptidase T1A family, specifically a 20S core alpha subunit. Two alternative transcripts, responsible for encoding different isoforms, have been identified.
Description
Recombinant human PSMA3, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 275 amino acids (residues 1-255) with a molecular weight of 30.6 kDa. This protein features a 20 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile filtered.
Formulation
The provided PSMA3 solution (concentration: 1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 1mM DTT, and 10% glycerol.
Stability
For optimal storage, refrigerate at 4°C if the entire vial will be used within 2-4 weeks. For long-term storage, freeze at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for extended storage durations. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 95% as determined by SDS-PAGE analysis.
Synonyms
proteasome (prosome, macropain) subunit alpha type-3, HC8, Macropain subunit C8, Multicatalytic endopeptidase complex subunit C8, Proteasome component C8, PSC3, PSC8, EC 3.4.25.1.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MSSIGTGYDL SASTFSPDGR VFQVEYAMKA VENSSTAIGI RCKDGVVFGV EKLVLSKLYE EGSNKRLFNV DRHVGMAVAG LLADARSLAD IAREEASNFR SNFGYNIPLK HLADRVAMYV HAYTLYSAVR PFGCSFMLGS YSVNDGAQLY MIDPSGVSYG YWGCAIGKAR QAAKTEIEKL QMKEMTCRDI VKEVAKIIYI VHDEVKDKAF ELELSWVGEL TNGRHEIVPK DIREEAEKYA KESLKEEDES DDDNM

Product Science Overview

Introduction

Proteasome Subunit Alpha Type 3, also known as PSMA3, is a crucial component of the proteasome complex in humans. This protein is encoded by the PSMA3 gene and plays a significant role in the degradation of intracellular proteins. The proteasome complex is essential for maintaining cellular homeostasis by regulating the concentration of specific proteins and degrading misfolded proteins.

Structure and Function

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of four rings of 28 non-identical subunits: two rings of seven alpha subunits and two rings of seven beta subunits . PSMA3 is one of the alpha subunits and is involved in the assembly and structural integrity of the proteasome complex .

The primary function of the proteasome is to degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds. This process is ATP-dependent and occurs in a non-lysosomal pathway. The proteasome complex plays numerous essential roles within the cell, including the regulation of the cell cycle, modulation of various signaling pathways, and the immune response .

Biological Processes

PSMA3 is involved in several critical biological processes, including:

  • Regulation of cellular amino acid metabolic process
  • Antigen processing and presentation of exogenous peptide antigen via MHC class I
  • Ubiquitin-dependent protein catabolic process
  • Positive regulation of canonical Wnt signaling pathway
  • Proteolysis involved in cellular protein catabolic process
Clinical Significance

Mutations or dysregulation of the PSMA3 gene can lead to various diseases. For instance, PSMA3 has been associated with cyclic neutropenia and dyskeratosis congenita, autosomal dominant 6 . Additionally, the proteasome’s role in degrading misfolded proteins makes it a target for therapeutic interventions in diseases characterized by protein aggregation, such as neurodegenerative disorders.

Research and Applications

Recombinant PSMA3 is used in research to study the proteasome’s structure and function. It is also utilized in drug discovery and development, particularly in identifying and testing proteasome inhibitors. These inhibitors have therapeutic potential in treating cancers and other diseases where proteasome activity is dysregulated .

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