Omentin Human, His

Omentin Human Recombinant, His Tag
Cat. No.
BT24906
Source
Escherichia Coli.
Synonyms
Intelectin-1, HL1, LFR, HL-1, INTL, ITLN, hIntL.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Omentin Human Recombinant is produced in E.Coli by recombinant DNA technology is a single, polypeptide chain containing 294 amino acids and having a molecular mass of 32.7 kDa.
Recombinant Human Omentin contains His tag fused at N-Terminus.
Intelectin is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Omentin, primarily found in visceral adipose tissue, is a recently discovered gene with significant presence in the tissue's stromal vascular cells, not adipocytes. Showing 150 times higher expression in visceral compared to subcutaneous adipose tissue, omentin has been identified in human blood through western blot analysis. Studies suggest its potential in increasing insulin-stimulated glucose uptake in mice adipocytes, particularly by enhancing Akt phosphorylation regardless of insulin presence. Although its exact role in glucose metabolism and obesity needs further clarification, an insulin-sensitizing effect is plausible. Notably, omentin expression levels vary between adipose tissue from healthy individuals and those with inflammatory bowel disease, but the implications remain unclear.
Description
Recombinant Human Omentin, a 32.7 kDa single polypeptide chain consisting of 294 amino acids, is produced in E. coli using recombinant DNA technology. This protein includes a His tag at the N-terminus and undergoes purification through proprietary chromatographic methods.
Physical Appearance
White, lyophilized (freeze-dried) powder, sterile filtered.
Formulation
Each milligram of lyophilized powder contains 20mM Tris and 50mM NaCl at a pH of 8.0.
Solubility
To reconstitute the lyophilized Omentin His Tag, it is recommended to dissolve it in sterile 18 megaohm-centimeter H2O at a concentration not lower than 100 micrograms per milliliter. This solution can then be diluted further into other aqueous solutions as needed.
Stability
While lyophilized Intelectin His Tag remains stable at room temperature for up to 3 weeks, it should be stored in dry conditions below -18 degrees Celsius. Once reconstituted, Intelectin should be kept at 4 degrees Celsius for a period of 2 to 7 days. For long-term storage, freezing below -18 degrees Celsius is advised. To preserve protein stability during storage, consider adding a carrier protein such as 0.1% HSA or BSA. Avoid repeated freeze-thaw cycles.
Purity
The purity is determined to be greater than 95.0% through the following analyses: (a) Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) and (b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE).
Applications
This product is suitable for use in Enzyme-Linked Immunosorbent Assay (ELISA) and Western blot applications.
Synonyms
Intelectin-1, HL1, LFR, HL-1, INTL, ITLN, hIntL.
Source
Escherichia Coli.
Amino Acid Sequence
MRGSHHHHHH GMASTDEANT YFKEWTCSSS PSLPRSCKEI KDECPSAFDG LYFLRTENGV IYQTFCDMTS GGGGWTLVAS VHENDMRGKC TVGDRWSSQQ GSKAVYPEGD GNWANYNTFG SAEAATSDDY KNPGYYDIQA KDLGIWHVPN KSPMQHWRNS SLLRYRTDTG FLQTLGHNLF GIYQKYPVKY GEGKCWTDNG PVIPVVYDFG DAQKTASYYS PYGQREFTAG FVQFRVFNNE RAANALCAGM RVTGCNTEHH CIGGGGYFPE ASPQQCGDFS GFDWSGYGTH VGYS.

Product Science Overview

Molecular Structure and Function

Omentin-1 contains a fibrinogen-like domain, which is crucial for its biological activity . It interacts with integrin receptors αvβ3 and αvβ5, modulating macrophage function and reversing plaque vulnerability in animal models of atherosclerosis . This interaction is essential for its role in cardiovascular health, as it suppresses inflammatory cytokines expression, macrophage infiltration, and apoptosis within atherosclerotic plaques .

Role in Cardiomyocyte Maturation

Omentin-1 is also involved in cardiomyocyte (CM) maturation during postnatal heart development . It facilitates CM cell cycle arrest and metabolic maturation by interacting with bone morphogenetic protein 7 (BMP7) . Deletion of omentin-1 in mice leads to heart enlargement and dysfunction in adulthood, highlighting its importance in maintaining cardiac function .

Human Recombinant Omentin (His Tag)

The recombinant form of omentin, tagged with a histidine (His) tag, is used in research to study its functions and interactions. The His tag allows for easy purification and detection of the protein in experimental settings. This recombinant protein is crucial for understanding the detailed mechanisms of omentin’s actions and its potential therapeutic applications.

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