Omentin 298 a.a. Human

Omentin is a single, non-glycosylated polypeptide chain consisting of 298 amino acids, with a molecular mass of approximately 33.2 kDa . The protein is produced in Escherichia coli (E. coli) and is purified using proprietary chromatographic techniques . The amino acid sequence of Omentin includes regions that are crucial for its biological activity, such as the ability to increase insulin-stimulated glucose uptake .

Omentin is predominantly expressed in visceral adipose tissue rather than subcutaneous adipose tissue . It is found in the stromal vascular cells within the adipose tissue, rather than in the adipocytes themselves . This localization is significant because visceral fat is closely associated with metabolic diseases such as diabetes and cardiovascular diseases.

One of the key functions of Omentin is its role in glucose metabolism. It has been shown to increase insulin-stimulated glucose uptake in adipocytes, which suggests that it may have an insulin-sensitizing effect . Additionally, Omentin has been observed to increase Akt phosphorylation, a process that is crucial for glucose uptake and metabolism .

The recombinant form of Omentin is produced in E. coli and is supplied as a sterile, filtered colorless solution . The protein is formulated in a buffer containing 20mM Tris-HCL (pH 8), 0.4M Urea, and 10% Glycerol . For optimal stability, it is recommended to store the protein at 4°C if used within 2-4 weeks, or at -20°C for longer periods. Adding a carrier protein, such as 0.1% HSA or BSA, can further enhance its stability .

Omentin’s role in glucose metabolism and its potential insulin-sensitizing effects make it a promising target for research into metabolic disorders such as diabetes and obesity . Differences in Omentin expression have also been noted in patients with inflammatory bowel disease, although the significance of these findings is still under investigation .