Omentin Human
Greater than 95.0% as determined by SDS-PAGE.
Description
35 kDa. Intelectin is purified by proprietary chromatographic techniques.
Product Specs
Omentin, primarily found in visceral adipose tissue, is a recently discovered gene localized to the mental tissue. Its presence is observed in the stromal vascular cells of adipose tissue, rather than in adipocytes. Notably, omentin exhibits significantly higher expression in visceral adipose tissue compared to subcutaneous tissue, with a 150-fold difference in mRNA levels. Studies using western blot analysis have confirmed the presence of omentin in human blood. Furthermore, research suggests that omentin may enhance insulin-stimulated glucose uptake in 3T3-L1 adipocytes in mice. It appears that omentin promotes Akt phosphorylation, regardless of insulin presence. While its precise role in glucose metabolism and obesity is yet to be fully elucidated, there is a possibility that it possesses insulin-sensitizing properties. Interestingly, variations in omentin expression have been observed between adipose tissue from healthy individuals and patients with inflammatory bowel disease, although the clinical significance of this finding remains unclear.
Recombinant Human Omentin is produced in E. coli using recombinant DNA technology. It is a single polypeptide chain comprising 313 amino acids, resulting in a molecular weight of 35 kDa. The purification process of Intelectin involves proprietary chromatographic techniques.
The product appears as a sterile, white powder that has been lyophilized (freeze-dried).
Each milligram of the lyophilized powder contains 10mM NaP (pH 7.5) and mannitol in a 5:1 ratio to the protein.
To reconstitute the lyophilized Omentin, it is recommended to dissolve it in sterile 18MΩ-cm H2O at a concentration of at least 100µg/ml. This solution can then be further diluted in other aqueous solutions as needed.
While lyophilized Intelectin can remain stable at room temperature for up to 3 weeks, it is recommended to store it desiccated at a temperature below -18°C. Once reconstituted, Intelectin should be stored at 4°C for a period of 2-7 days. For long-term storage, it is advisable to freeze it below -18°C. To enhance stability during long-term storage, consider adding a carrier protein such as HSA or BSA (0.1%). Avoid repeated freeze-thaw cycles to maintain product integrity.
SDS-PAGE analysis indicates that the purity of this product is greater than 95%.
Product Science Overview
Omentin is predominantly expressed in visceral adipose tissue rather than subcutaneous tissue. The mRNA levels of omentin are significantly higher in visceral adipose tissue, being approximately 150 times higher compared to subcutaneous tissue . This protein is present in the stromal vascular cells within adipose tissue, rather than in adipocytes themselves .
Omentin enhances insulin-stimulated glucose uptake in adipocytes, making it a crucial link between obesity and Type 2 Diabetes . It has been shown to play a protective role in the response to cardiovascular injury . Additionally, omentin interacts with bone morphogenetic protein 7 (BMP7) to regulate cardiomyocyte (CM) maturation. This interaction prevents BMP7 from binding to activin type II receptor B (ActRIIB), subsequently decreasing the downstream pathways involving mothers against DPP homolog 1 (SMAD1)/Yes-associated protein (YAP) and p38 mitogen-activated protein kinase (p38 MAPK) .
Recombinant human omentin is produced using advanced biotechnological methods. It is typically derived from a mouse myeloma cell line (NS0) and is purified to a high degree, with a purity greater than 95% as determined by SDS-PAGE . The recombinant protein is often used in research to study its effects on various cell types and its potential therapeutic applications.
The role of omentin in enhancing insulin sensitivity and its protective effects on cardiovascular tissues make it a potential therapeutic target for treating metabolic disorders such as obesity and Type 2 Diabetes. Furthermore, its involvement in cardiomyocyte maturation suggests that it could be important in developing treatments for heart diseases .
