MIP 3a Human, His

Macrophage Inflammatory protein-3 alpha (CCL20) Human Recombinant, His Tag
Cat. No.
BT20958
Source
Escherichia Coli.
Synonyms
S Small inducible cytokine A20 precursor, CCL20, Macrophage inflammatory protein 3 alpha, MIP-3-alpha, Liver and activation- regulated chemokine, CC chemokine LARC, Beta chemokine exodus-1, CKb4, LARC, ST38, MIP3A, MIP-3a, SCYA20.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

MIP 3a Human Recombinant fused with a 21 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 91 amino acids (27-96 a.a.) and having a molecular mass of 10.3kDa. The MIP 3a is purified by proprietary chromatographic techniques.

Product Specs

Introduction
CCL-20, also known as MIP-3 alpha, is a chemotactic factor that primarily attracts lymphocytes and neutrophils, rather than monocytes. It plays a crucial role in the immune response by directing immune cells to sites of inflammation or infection. CCL-20 inhibits the proliferation of myeloid progenitor cells and contributes to the formation and function of mucosal lymphoid tissues by attracting lymphocytes and dendritic cells. This chemokine exhibits antibacterial activity against E. coli and S. aureus. CCL-20 gene expression is activated by H. pylori infection, leading to the recruitment of immune cells to the gastric mucosa. It is also involved in the recruitment of T cells to the intestinal lining and the activation of Langerhans cells in the vaginal epithelium, potentially playing a role in HIV transmission.
Description
Recombinant human MIP-3 alpha (CCL-20) is a 10.3 kDa protein engineered with a 21 amino acid His tag at the N-terminus. It is expressed in E. coli and purified as a single, non-glycosylated polypeptide chain encompassing amino acids 27-96 of the mature protein.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The MIP-3 alpha protein is supplied at a concentration of 0.25 mg/ml in a solution of Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For long-term storage, it is recommended to store the protein at -20°C. To ensure stability during long-term storage, the addition of a carrier protein such as HSA or BSA (0.1%) is advised. Repeated freezing and thawing of the product should be avoided.
Purity
The purity of the MIP-3 alpha protein is determined to be greater than 85% using SDS-PAGE analysis.
Synonyms
S Small inducible cytokine A20 precursor, CCL20, Macrophage inflammatory protein 3 alpha, MIP-3-alpha, Liver and activation- regulated chemokine, CC chemokine LARC, Beta chemokine exodus-1, CKb4, LARC, ST38, MIP3A, MIP-3a, SCYA20.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MASNFDCCLG YTDRILHPKF IVGFTRQLAN EGCDINAIIF HTKKKLSVCA NPKQTWVKYI VRLLSKKVKN M.

Product Science Overview

Expression and Regulation

CCL20 is expressed in various tissues, including the liver, lungs, lymph nodes, and peripheral blood lymphocytes . Its expression is strongly upregulated by inflammatory signals and downregulated by the anti-inflammatory cytokine IL-10 . This regulation ensures that CCL20 is produced in response to inflammation, aiding in the recruitment of immune cells to the site of infection or injury.

Function and Mechanism

CCL20 exerts its effects by binding to the G protein-coupled receptor CCR6 . This interaction is crucial for the chemoattraction of lymphocytes and dendritic cells towards epithelial cells, particularly in mucosal tissues . The protein is strongly chemotactic for lymphocytes and weakly attracts neutrophils . It is also involved in the formation and function of mucosal lymphoid tissues, which are essential for the body’s immune defense .

Recombinant Human CCL20

Recombinant human CCL20 is a non-glycosylated protein consisting of 70 amino acids and has a molecular weight of approximately 8.0 kDa . The recombinant form is often tagged with a His (histidine) tag to facilitate purification and detection in research applications. This form is widely used in scientific studies to understand the protein’s function and its role in various biological processes.

Applications in Research

Recombinant CCL20 is primarily used for research purposes, including studies on immune response, inflammation, and cell signaling. It is not intended for human, animal, or diagnostic applications . Researchers utilize this protein to investigate its role in attracting immune cells and its potential therapeutic applications in treating inflammatory diseases.

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