MIP 3 Human
Macrophage Inflammatory Protein-3 Human Recombinant (CCL23)
Cat. No.
BT20781
Source
Escherichia Coli.
Synonyms
C-C motif chemokine 23, Small-inducible cytokine A23, Macrophage inflammatory protein 3, Myeloid progenitor inhibitory factor 1, CK-beta-8, MIP-3, MPIF-1, CKB-8, CCL23, MIP3, MPIF1, SCYA23, CKb8, Ckb-8-1.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
MIP-3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 99 amino acids and having a molecular mass of 11.3kDa.
The MIP-3 is purified by proprietary chromatographic techniques.
The MIP-3 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
CCL23 (MIP-3) is a chemokine that binds to the CCR1 receptor. The gene for CCL23 is located on chromosome 17q, in a region that contains a cluster of CC chemokine genes. MIP-3 can attract monocytes, resting T lymphocytes, and neutrophils but not activated lymphocytes. It has also been shown to inhibit colony formation by immature myeloid progenitors from bone marrow. MIP-3 is primarily expressed in the lung and liver but can also be found in bone marrow, placenta, and some myeloid cell lines. Two isoforms of MIP-3 are produced through alternative splicing of the CCL23 gene, resulting in a short (CK8) and a long (CK81) isoform. The CK8 cDNA encodes a 120-amino acid precursor protein that contains a putative 21 amino acid signal peptide. Cleavage of this signal peptide generates a mature 99 amino acid CK8 protein (amino acids 22-120). Further N-terminal processing can produce a 75-amino acid CK8 protein (amino acids 46-120) which is significantly more active than the 99-amino acid variant. MIP-3 may contribute to the progression of certain cancers that overexpress ErbB2 by transactivating ErbB2 tyrosine kinase. MIP-3 might also be involved in angiogenesis by upregulating matrix metalloproteinase MMP-2 expression.
Description
Recombinant human MIP-3, produced in E. coli, is a single, non-glycosylated polypeptide chain containing 99 amino acids with a molecular weight of 11.3 kDa. The MIP-3 protein is purified using proprietary chromatographic techniques.
Physical Appearance
Sterile white lyophilized powder.
Formulation
Lyophilized from a 1 mg/ml solution in 20 mM phosphate buffer (PB), pH 7.4, 150 mM NaCl, and filtered through a 0.2 µm filter.
Solubility
It is recommended to first reconstitute the lyophilized MIP-3 in sterile 18 MΩ-cm H2O to a concentration of no less than 100 µg/ml. The reconstituted solution can then be diluted into other aqueous solutions.
Stability
Lyophilized MIP-3 can be stored at room temperature for 3 weeks but is best stored desiccated below -18°C. Reconstituted CCL23 should be stored at 4°C for 2-7 days or at -18°C for longer-term storage. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid freeze-thaw cycles.
Purity
Greater than 97.0% pure as determined by: (a) RP-HPLC analysis and (b) SDS-PAGE analysis.
Biological Activity
The biological activity is determined based on the ability to chemoattract human T cells. The activity is measured using a concentration range of 10-50 ng/ml, corresponding to a specific activity of 20,000-100,000 IU/mg.
Synonyms
C-C motif chemokine 23, Small-inducible cytokine A23, Macrophage inflammatory protein 3, Myeloid progenitor inhibitory factor 1, CK-beta-8, MIP-3, MPIF-1, CKB-8, CCL23, MIP3, MPIF1, SCYA23, CKb8, Ckb-8-1.
Source
Escherichia Coli.
Amino Acid Sequence
RVTKDAETEF MMSKLPLENP VLLDRFHATS ADCCISYTPR SIPCSLLESYFETNSECSKP GVIFLTKKGR RFCANPSDKQ VQVCMRMLKL DTRIKTRKN.
Product Science Overview
Gene and Expression
Function and Activity
Recombinant CCL23
Biological Implications
Stability and Storage
Lyophilized CCL23 is stable at room temperature for up to three weeks but should be stored desiccated below -18°C for long-term storage . Upon reconstitution, it should be stored at 4°C for short-term use and below -18°C for long-term use, with the addition of a carrier protein to prevent freeze-thaw cycles .
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