MIP 3b Human

Macrophage Inflammatory Protein-3 Beta Human Recombinant (CCL19)
Cat. No.
BT21113
Source
Escherichia Coli.
Synonyms
Small inducible cytokine A19, CCL19, Macrophage inflammatory protein 3 beta, MIP-3- beta, EBI1-ligand chemokine, ELC, Beta chemokine exodus-3, CK beta-11, chemokine (C-C motif) ligand 19, CKb11, MIP3B, MIP-3b, SCYA19, MGC34433.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98.0% as determined by
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
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Description

Macrophage Inflammatory Protein-3 beta Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 77 amino acids and having a molecular mass of 8.8kDa.
The MIP-3b is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Chemokine (C-C motif) ligand 19 (CCL19), also known as EBI1 ligand chemokine (ELC) or macrophage inflammatory protein-3-beta (MIP-3-beta), is a small cytokine classified as a CC chemokine. This chemokine is primarily found in the thymus and lymph nodes, with significant presence in the trachea and colon, and lower levels in organs such as the stomach, small intestine, lung, kidney, and spleen. The gene encoding CCL19 is situated on human chromosome 9. CCL19 exerts its effects by binding to the chemokine receptor CCR7, attracting immune cells like dendritic cells and antigen-activated B cells.
Description
Recombinant Human Macrophage Inflammatory Protein-3 beta, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 77 amino acids. With a molecular weight of 8.8 kDa, this protein is purified using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
Lyophilized without any additional ingredients.
Solubility
To reconstitute the lyophilized Macrophage Inflammatory Protein-3b, it is advised to dissolve it in sterile 18MΩ-cm H2O at a minimum concentration of 100µg/ml. This solution can be further diluted with other aqueous solutions as needed.
Stability
Lyophilized MIP-3b, though stable at room temperature for up to 3 weeks, should ideally be stored in a dry environment below -18°C. After reconstitution, CCL19 should be stored at 4°C for a period of 2-7 days. For extended storage, freezing below -18°C is recommended. To ensure long-term stability during storage, adding a carrier protein like 0.1% HSA or BSA is advisable. It is essential to minimize freeze-thaw cycles.
Purity
Exceeding 98.0% as determined by (a) RP-HPLC analysis and (b) SDS-PAGE analysis.
Biological Activity
CCL19's activity is assessed by its chemoattractant effect on human T cells. Using a concentration range of 7-40 ng/ml, the specific activity is determined to be between 25,000 and 143,000 IU/mg.
Synonyms
Small inducible cytokine A19, CCL19, Macrophage inflammatory protein 3 beta, MIP-3- beta, EBI1-ligand chemokine, ELC, Beta chemokine exodus-3, CK beta-11, chemokine (C-C motif) ligand 19, CKb11, MIP3B, MIP-3b, SCYA19, MGC34433.
Source
Escherichia Coli.
Amino Acid Sequence
GTNDAEDCCL SVTQKPIPGY IVRNFHYLLI KDGCRVPAVV FTTLRGRQLC APPDQPWVER IIQRLQRTSA KMKRRSS.

Product Science Overview

Structure and Expression

CCL19 is characterized by the presence of four highly conserved cysteine residues, which are typical of CC chemokines . The human recombinant form of CCL19 is an 8.8 kDa protein consisting of 77 amino acid residues . It is expressed primarily in the thymus, lymph nodes, and activated bone marrow stromal cells .

Function and Mechanism

CCL19 functions by binding to the G protein-coupled receptor CCR7, which is expressed on T cells and mature dendritic cells . This interaction is critical for the homeostatic trafficking of naive T cells and activated dendritic cells into secondary lymphoid organs . CCL19, along with its closely related chemokine CCL21, plays a significant role in T cell priming and activation, as well as the recruitment of lymphocytes to inflamed tissues .

Biological Activity

The biological activity of CCL19 includes its ability to chemoattract human T cells at concentrations ranging from 10.0 to 50.0 ng/ml . This chemotactic property is essential for the regulation of lymphocyte circulation and homing in the thymus and secondary lymphoid organs . Additionally, CCL19 is involved in the migration of antigen-presenting cells, such as dendritic cells and antigen-loaded B cells .

Applications in Research

Human recombinant CCL19 is widely used in research for various applications, including:

  • Chemotactic assays: Studying the migration of lymphoid and myeloid cells .
  • Dendritic cell maturation: Inducing the maturation of dendritic cells and T cell proliferation .
  • Functional assays: Investigating the functional roles of CCL19 in immune responses .

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