MIP 3b Human, T7
Description
MIP3b is fused to a 16 amino acid T7-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Macrophage Inflammatory Protein-3 (MIP-3), also known as CCL19, is a member of the CC chemokine family. Chemokines are small cytokines or signaling proteins secreted by cells that influence the immune system by inducing chemotaxis in nearby responsive cells. CCL19, in particular, plays a crucial role in the immune response by guiding the migration of immune cells to sites of inflammation or injury.
CCL19 is a protein that consists of 93 amino acids and has a molecular mass of approximately 10.4 kDa . It is closely related to another chemokine, CCL21, sharing about 32% amino acid sequence homology . Despite this similarity, CCL19 and CCL21 have distinct expression patterns and functions. CCL19 is primarily expressed in the T cell-rich zones of lymph nodes and is involved in the migration of T cells, B cells, and dendritic cells .
CCL19 exerts its effects by binding to the C-C motif chemokine receptor 7 (CCR7) . This interaction induces the migration of immune cells, such as macrophages, T cells, and B cells, to lymphoid tissues where they can initiate an immune response . Additionally, CCL19 has been shown to bind to other receptors, such as CCX-CKR and CRAM-B, although the functional significance of these interactions is less well understood .
Recombinant CCL19 is produced using recombinant DNA technology, which involves inserting the gene encoding CCL19 into a suitable expression system, such as E. coli. The recombinant protein is then purified using chromatographic techniques to ensure high purity and activity . The addition of a T7 tag, a short peptide sequence, facilitates the detection and purification of the recombinant protein.
Recombinant CCL19 is widely used in research to study its role in immune cell migration and its potential therapeutic applications. It is particularly valuable in experiments investigating the mechanisms of immune cell trafficking and the development of immune responses. Additionally, recombinant CCL19 can be used in drug development to identify potential targets for immunomodulatory therapies.
