MIP 3b Mouse

CCL19 is a small protein with a molecular weight of approximately 9.2 kDa . It contains 83 amino acid residues, including four highly conserved cysteine residues characteristic of CC chemokines . The protein is expressed in various tissues, including the thymus, lymph nodes, and activated bone marrow stromal cells .

CCL19 signals through the G protein-coupled receptor CCR7, which is expressed on T cells and mature dendritic cells . This signaling pathway is essential for the homeostatic trafficking of naive T cells and activated dendritic cells into secondary lymphoid organs . Additionally, CCL19 plays a role in T cell priming and activation, as well as the recruitment of lymphocytes to inflamed tissues .

The biological activity of CCL19 includes its ability to chemoattract activated human T cells expressing the CCR7 receptor . This chemotactic activity is crucial for the proper functioning of the immune system, as it ensures that immune cells are correctly positioned within the body to respond to infections and other immune challenges.

Recombinant CCL19 is produced using various expression systems, including E. coli and mammalian cells . The recombinant protein is typically purified to a high degree of purity (≥ 98%) and is free from endotoxins . It is available in various forms, including lyophilized and carrier-free formulations .

Recombinant CCL19 is widely used in research to study immune cell trafficking, T cell activation, and dendritic cell function. It is also used in various assays, including immunohistochemistry, ELISA, and functional assays .

Recombinant CCL19 should be stored at -20°C for long-term storage and can be shipped at ambient temperature . For maximum stability, it is recommended to avoid repeated freeze-thaw cycles and to aliquot the protein for single-use applications .