Introduction
CCL20, also known as MIP-3 alpha, is a chemotactic factor that primarily attracts lymphocytes and neutrophils, rather than monocytes. It plays a crucial role in the immune system by guiding these cells to sites of inflammation and infection. MIP-3 alpha has been shown to inhibit the proliferation of myeloid progenitor cells and is involved in the development and function of mucosal lymphoid tissues. This chemokine attracts lymphocytes and dendritic cells to epithelial cells, contributing to immune surveillance at mucosal surfaces. CCL20 exhibits antibacterial activity against E. coli and S. aureus. Notably, the bacterium H. pylori can activate CCL20 gene transcription through intracellular signaling pathways involving NF-kappaB. MIP-3 alpha is also involved in chemokine-mediated lymphocyte trafficking during gastric inflammation caused by Helicobacter infection. Furthermore, CCL20 expression is implicated in the recruitment of specific T cell subsets into the intestinal lining. In addition to its roles in the gastrointestinal tract, MIP-3 alpha contributes to pulpal inflammation by recruiting lymphocytes expressing the C-C motif Receptor 6. Vaginal epithelial cells secrete MIP-3 alpha in response to factors present in semen, leading to increased recruitment of Langerhans cells, which may have implications for HIV transmission.
Description
Macrophage Inflammatory Protein-3 alpha Mouse Recombinant, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 70 amino acids. It has a molecular mass of 7.9 kDa. The purification process for MIP-3a involves proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a concentrated (1mg/ml) solution in water without any additives.
Solubility
To reconstitute the lyophilized MIP-3a, it is recommended to dissolve it in sterile 18 MΩ-cm H2O at a concentration of at least 100 µg/ml. This solution can then be further diluted in other aqueous solutions as needed.
Stability
Lyophilized MIP-3a remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store it desiccated at a temperature below -18°C. After reconstitution, CCL-20 should be stored at 4°C for 2-7 days. For extended storage, it is advisable to freeze it at -18°C. To preserve its stability during long-term storage, consider adding a carrier protein (0.1% HSA or BSA). It's important to avoid repeated freeze-thaw cycles.
Purity
The purity of MIP-3a is determined using two methods: RP-HPLC analysis and SDS-PAGE analysis. Both methods consistently show a purity greater than 95.0%.
Biological Activity
The biological activity of MIP-3a is determined by its capacity to attract primary human T cells. It exhibits chemoattractant activity at concentrations ranging from 2 to 20 ng/ml. Based on this activity, the specific activity of MIP-3a is estimated to be between 50,000 and 500,000 units/mg.
Synonyms
Small inducible cytokine A20 precursor, CCL20, Macrophage inflammatory protein 3 alpha, MIP-3-alpha, Liver and activation- regulated chemokine, CC chemokine LARC, Beta chemokine exodus-1, CKb4, LARC, ST38, MIP3A, MIP-3a, SCYA20, C-C motif chemokine 20, CC chemokine ST38, exodus-1.
Amino Acid Sequence
ASNYDCCLSY IQTPLPSRAI VGFTRQMADE ACDINAIIFH TKKRKSVCAD PKQNWVKRAV NLLSLRVKKM.
