MIP 3a Human
Macrophage Inflammatory Protein-3 alpha Human Recombinant (CCL20)
Cat. No.
BT20875
Source
Escherichia Coli.
Synonyms
S Small inducible cytokine A20 precursor, CCL20, Macrophage inflammatory protein 3 alpha, MIP-3-alpha, Liver and activation- regulated chemokine, CC chemokine LARC, Beta chemokine exodus-1, CKb4, LARC, ST38, MIP3A, MIP-3a, SCYA20.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
MIP-3 Alpha Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 8 kDa.
The MIP-3a is purified by proprietary chromatographic techniques.
The MIP-3a is purified by proprietary chromatographic techniques.
Product Specs
Introduction
CCL-20, also known as MIP-3 alpha, is a chemotactic factor that attracts lymphocytes and neutrophils, but not monocytes. It inhibits the proliferation of myeloid progenitor cells and plays a crucial role in the development and function of mucosal lymphoid tissues by directing lymphocytes and dendritic cells toward epithelial cells. Notably, C-terminal processed forms of CCL-20 exhibit equivalent chemotactic activity on leukocytes. CCL-20 also possesses antibacterial properties against E. coli (ATCC 25922) and S. aureus (ATCC 29213). The transcription of the CCL-20 gene is activated by H. pylori, which in turn activates NF-kappaB via an intracellular signaling cascade involving IkappaB kinase and NF-kappaB-inducing kinase. MIP-3 alpha is implicated in chemokine-mediated lymphocyte trafficking during gastric inflammation induced by Helicobacter infection. CCL-20 expression contributes to the recruitment of CD45R0-positive T cell subsets to the intestinal lamina propria. Furthermore, MIP-3A promotes pulpal inflammation by recruiting C-C motif Receptor 6-expressing lymphocytes. Vaginal epithelial cells, in response to factors present in semen, secrete MIP-3 alpha, thereby enhancing Langerhans cell recruitment during HIV transmission.
Description
Recombinant Human MIP-3 Alpha, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 70 amino acids. It has a molecular weight of 8 kDa. The purification process of MIP-3a involves proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
Lyophilized from a 0.2 µm filtered solution in 20mM Phosphate Buffer (PB), pH 7.4, containing 150mM Sodium Chloride (NaCl).
Solubility
To reconstitute the lyophilized MIP-3 Alpha, it is recommended to dissolve it in sterile 18 MΩ-cm H2O at a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized MIP-3a remains stable at room temperature for up to 3 weeks; however, it is recommended to store it desiccated at a temperature below -18°C. After reconstitution, CCL20 should be stored at 4°C for 2-7 days. For long-term storage, it is recommended to store it below -18°C. To ensure optimal stability during long-term storage, consider adding a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Purity
The purity of MIP-3a is greater than 97.0%, as determined by: (a) Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) analysis. (b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis.
Biological Activity
The biological activity of MIP-3a is assessed by its chemotactic effect on human T lymphocytes. This is determined using a concentration range of 10.0 - 50.0 ng/ml, which corresponds to a specific activity of 20,000 - 100,000 units/mg.
Synonyms
S Small inducible cytokine A20 precursor, CCL20, Macrophage inflammatory protein 3 alpha, MIP-3-alpha, Liver and activation- regulated chemokine, CC chemokine LARC, Beta chemokine exodus-1, CKb4, LARC, ST38, MIP3A, MIP-3a, SCYA20.
Source
Escherichia Coli.
Amino Acid Sequence
ASNFDCCLGY TDRILHPKFI VGFTRQLANE GCDINAIIFH TKKKLSVCAN PKQTWVKYIV RLLSKKVKNM.
Product Science Overview
Expression and Regulation
Structure and Molecular Characteristics
Human recombinant CCL20 is a protein consisting of 70 amino acids with a molecular weight of approximately 8 kDa . It contains four highly conserved cysteine residues, which are characteristic of CC chemokines . The protein is typically produced in an E. coli expression system and purified to a high degree of purity (>98%) for research purposes .
Function and Mechanism of Action
CCL20 signals through the G protein-coupled receptor CCR6 . It acts as a chemoattractant, primarily attracting lymphocytes and dendritic cells to sites of inflammation . This chemokine is particularly important in mucosal immunity, where it helps to recruit immune cells to the mucosal surfaces of the gut, lungs, and skin .
Clinical and Research Applications
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
