MHC Class I, H-2K Antibody, FITC

MHC Class I, (H-2K) Mouse Antibody, FITC
Cat. No.
BT25231
Source
Synonyms
Appearance
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

Product Specs

Introduction
MHC Class I is a transmembrane protein complex crucial for the adaptive immune response. It comprises two polypeptide chains: a larger alpha chain and a smaller beta-2 microglobulin. The alpha chain folds into three domains (Alpha-1, Alpha-2, Alpha-3) with the Alpha-3 domain anchoring the complex to the cell membrane. The peptide-binding groove, situated between the Alpha-1 and Alpha-2 domains, accommodates short peptide fragments derived from intracellular proteins. This peptide-MHC complex is presented on the cell surface for surveillance by cytotoxic T lymphocytes (CTLs). The diverse repertoire of peptides presented by MHC Class I molecules ensures broad antigen recognition. The interaction between the peptide-MHC complex and the T cell receptor (TCR) on CTLs is characterized by a balance between specificity and affinity, essential for effective immune surveillance.
Formulation
Supplied as a 1 mg/ml solution in phosphate-buffered saline (PBS).
Storage Procedures
The lyophilized antibody should be stored at 4 degrees Celsius. After reconstitution, it is recommended to use the antibody promptly. For long-term storage, aliquot the reconstituted antibody and store at -20 degrees Celsius to preserve its activity.
Solubility
To reconstitute the lyophilized antibody, add deionized water. Gently mix the solution to ensure complete dissolution. Briefly centrifuge the vial to collect any droplets on the sides. Allow the reconstituted antibody to stand at room temperature for 30-60 seconds before use.
Applications
This antibody has applications in cytotoxicity assays and cell staining. For staining applications, use 10 microliters of antibody per 1 million cells. The appropriate titer for cytotoxicity assays needs to be empirically determined by the researcher.
Available Conjugates
In addition to the FITC-conjugated version, this antibody is also available in unconjugated and biotinylated formats.
Purification Method
Ion exchange column.
Specificity
Recognizes most mouse H-2K haplotypes (k,p,q,r,s). Does NOT react with H-2kd.
Type
Mouse Antibody Monoclonal.
Clone
NYRmH-2K.
Immunogen
Purified mouse LN cells (C57Bl/6 anti-BALB/c) .
Ig Subclass
Mouse IgG2b.

Product Science Overview

Introduction

MHC Class I molecules are essential components of the immune system, playing a crucial role in the presentation of intracellular antigens to cytotoxic T cells. The H-2K molecule is a specific type of MHC Class I molecule found in mice. The FITC-conjugated mouse antibody against H-2K is widely used in immunological research to study antigen presentation and immune responses.

MHC Class I Molecules

MHC Class I molecules are glycoproteins expressed on the surface of almost all nucleated cells. They present peptide fragments derived from intracellular proteins to CD8+ T cells. This process is vital for the immune system to recognize and eliminate infected or malignant cells. The MHC Class I molecule consists of a heavy chain, β2-microglobulin, and a peptide-binding groove that accommodates the antigenic peptide.

H-2K Molecule

The H-2K molecule is a specific MHC Class I molecule encoded by the H-2 complex in mice. It is involved in presenting endogenous antigens to CD8+ T cells, triggering an immune response. The H-2K molecule is polymorphic, meaning there are multiple alleles, each encoding a slightly different protein. This polymorphism is crucial for the immune system’s ability to recognize a wide range of antigens.

FITC Conjugation

Fluorescein isothiocyanate (FITC) is a fluorescent dye commonly used to label antibodies. FITC-conjugated antibodies are used in various applications, including flow cytometry and immunofluorescence microscopy, to detect specific antigens. The FITC label allows researchers to visualize and quantify the binding of the antibody to its target antigen.

Mouse Antibody Against H-2K

The mouse antibody against H-2K is a monoclonal antibody that specifically binds to the H-2K molecule. This antibody is used in research to study the expression and function of MHC Class I molecules in mice. The FITC-conjugated version of this antibody enables researchers to use fluorescence-based techniques to analyze H-2K expression.

Applications in Research

The FITC-conjugated mouse antibody against H-2K is used in various research applications, including:

  • Flow Cytometry: To analyze the expression of H-2K on the surface of cells.
  • Immunofluorescence Microscopy: To visualize the localization of H-2K in tissues or cells.
  • Immunohistochemistry: To study the distribution of H-2K in tissue sections.
  • Functional Assays: To investigate the role of H-2K in antigen presentation and immune responses.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

MHC Class II, I-A Antibody
MHC Class II, (I-A) Mouse Antibody
Cat. No.
BT25447
Source
MHC Class II, I-A Antibody, FITC
MHC Class II, (I-A) Mouse Antibody, FITC
Cat. No.
BT25621
Source
MHC CLASS II, I-E Antibody, Biotin
MHC CLASS II, (I-E) Mouse Antibody, Biotin
Cat. No.
BT25799
Source
MHC CLASS II, I-E Antibody, FITC
MHC CLASS II, (I-E) Mouse Antibody, FITC
Cat. No.
BT25892
Source
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.