MHC Class II, I-A Antibody, FITC
Description
Product Specs
Product Science Overview
The MHC Class II, (I-A) (Mouse Antibody, FITC) is a monoclonal antibody that targets the major histocompatibility complex (MHC) class II molecules, specifically the I-A and I-E subregion-encoded glycoproteins in mice. This antibody is conjugated with fluorescein isothiocyanate (FITC), a fluorescent dye, which allows for the visualization and analysis of MHC class II molecules using flow cytometry and other fluorescence-based techniques.
MHC class II molecules are essential for the immune system’s ability to recognize and respond to foreign antigens. They are primarily expressed on antigen-presenting cells (APCs) such as B cells, dendritic cells, macrophages, and a subset of activated T cells. These molecules present processed antigenic peptides to CD4+ T helper cells, initiating an immune response.
The MHC Class II, (I-A) (Mouse Antibody, FITC) specifically reacts with a polymorphic determinant shared by the I-A^b, I-A^d, I-A^q, I-E^d, and I-E^k MHC class II alloantigens. It does not react with I-A^f, I-A^k, or I-A^s alloantigens . This specificity makes it a valuable tool for studying the expression and function of MHC class II molecules in various mouse strains.
This antibody is widely used in immunological research for various applications, including:
- Flow Cytometry: The FITC conjugation allows for the detection and quantification of MHC class II molecules on the surface of cells using flow cytometry .
- Immunohistochemistry: It can be used to stain tissue sections to visualize the distribution of MHC class II molecules in different tissues .
- Immunoprecipitation: The antibody can be used to isolate MHC class II molecules from cell lysates for further analysis .
- Blocking Studies: It can be used to block MHC class II-mediated antigen presentation, helping to study the role of these molecules in immune responses .
The MHC Class II, (I-A) (Mouse Antibody, FITC) is typically purified by affinity chromatography and conjugated with FITC under optimal conditions. The antibody solution is formulated in a phosphate-buffered solution (pH 7.2) containing 0.09% sodium azide as a preservative. It should be stored undiluted between 2°C and 8°C and protected from prolonged exposure to light to maintain its stability and functionality .
