MHC CLASS II, I-E Antibody, FITC
Description
Product Specs
Product Science Overview
The Major Histocompatibility Complex (MHC) class II molecules are essential components of the immune system. They are heterodimeric transmembrane glycoproteins expressed on the surface of antigen-presenting cells (APCs) such as dendritic cells, macrophages, and B cells. These molecules play a crucial role in the presentation of extracellular antigens to CD4+ T cells, initiating immune responses.
MHC class II molecules are encoded by the I-A and I-E subregions of the MHC complex. The I-E molecules are polymorphic and are expressed on the surface of APCs. They are involved in presenting processed antigenic peptides to CD4+ T cells, which is critical for the activation of these T cells and the subsequent immune response.
The M5/114.15.2 monoclonal antibody specifically targets the MHC class II I-E molecules in mice. This antibody is derived from rat and belongs to the IgG2b isotype. It recognizes a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying specific haplotypes (H-2^b, H-2^d, H-2^q, H-2^p, H-2^r, and H-2^u) but not from mice carrying other haplotypes (H-2^s or H-2^f) .
Fluorescein isothiocyanate (FITC) is a fluorescent dye commonly used to label antibodies for flow cytometry and other fluorescence-based assays. The M5/114.15.2 antibody conjugated with FITC allows for the detection and analysis of MHC class II I-E molecules on the surface of cells using flow cytometry. This conjugation enables researchers to study the expression patterns and distribution of these molecules in various cell types and tissues .
The M5/114.15.2 antibody has been widely used in immunological research to study the role of MHC class II molecules in antigen presentation and T cell activation. It has been employed in various applications, including:
- Flow Cytometry: For the detection and quantification of MHC class II I-E molecules on the surface of cells.
- Immunohistochemistry: To visualize the distribution of these molecules in tissue sections.
- Functional Assays: To investigate the role of MHC class II molecules in immune responses and to block antigen presentation in vitro and in vivo .
