MHC Class II, I-A Antibody, Biotin

MHC Class II, (I-A) Mouse Antibody, Biotin
Cat. No.
BT25517
Source
Synonyms
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Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
MHC Class II is a heterodimeric protein complex composed of two transmembrane proteins, each approximately 30 kDa in size. Both proteins exhibit two globular domains: Alpha-1, Alpha-2, Beta-1, and Beta-2. The alpha-1 and beta-1 domains are positioned furthest from the cell membrane. These two proteins associate non-covalently. Primarily, MHC Class II molecules present peptides derived from exogenous sources. Expression of MHC Class II is typically restricted to Antigen Presenting Cells (APCs), which are responsible for the uptake and processing of foreign proteins. Within the endoplasmic reticulum (ER), the alpha and beta chains of MHC Class II associate. This association requires a third protein, the invariant chain, for stability. The MHC-invariant chain complex then traffics from the ER to the Golgi apparatus and back. Subsequently, it fuses with an endocytic compartment containing degraded fragments of internalized extracellular protein.
Formulation
Supplied as a 1 mg/ml solution in phosphate-buffered saline (PBS) following reconstitution.
Storage Procedures
Store the lyophilized product at 4°C. Upon reconstitution, aliquot the antibody if not intended for immediate use and store at -20°C to ensure long-term stability.
Solubility
To reconstitute, add deionized water to the vial. Gently mix the solution, ensuring the sides of the vial are washed. Allow 30-60 seconds for complete reconstitution before use.
Applications
This antibody is suitable for both cytotoxic and staining applications. For staining procedures, it is recommended to use 10 ml of antibody solution per 1,000,000 cells. The optimal titer for cytotoxicity assays should be determined empirically by the investigator.
Available Conjugates
In addition to the biotinylated format, this antibody is also offered in unconjugated and FITC-conjugated forms.
Purification Method
Ion exchange column.
Specificity
Recognizes most MHC class II haplotypes (b,f,p,q,r,s,u,v), but weak against I-Ak.
Type
Mouse Antibody Monoclonal.
Clone
NYRmI-A.
Immunogen
Purified mouse LN B cells (C3H anti-C57Bl6).
Ig Subclass
Mouse IgG2a.

Product Science Overview

Introduction

The Major Histocompatibility Complex (MHC) class II molecules are essential components of the immune system. They play a crucial role in the presentation of antigens to T cells, which is vital for initiating immune responses. The MHC class II molecules are primarily expressed on professional antigen-presenting cells (APCs) such as macrophages, dendritic cells, and B cells.

Structure and Function

MHC class II molecules are transmembrane glycoproteins composed of two non-covalently associated polypeptide chains, α and β. These molecules present processed peptide antigens derived from extracellular proteins to CD4+ T helper cells. This interaction is critical for the activation of T cells and the subsequent immune response.

The MHC class II molecules are encoded by genes located in the MHC region on chromosome 6 in humans and chromosome 17 in mice. In mice, the MHC class II region includes the I-A and I-E subregions, which encode the α and β chains of the MHC class II molecules.

MHC Class II (I-A) Mouse Antibody

The MHC Class II (I-A) mouse antibody, specifically the M5/114.15.2 clone, is a monoclonal antibody that reacts with the mouse MHC class II molecules encoded by the I-A and I-E subregions. This antibody is widely used in immunological research to study the expression and function of MHC class II molecules on various cell types.

The M5/114.15.2 antibody detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying specific haplotypes (H-2^b, H-2^d, H-2^q, H-2^p, H-2^r, and H-2^u) but not from mice carrying other haplotypes (H-2^s or H-2^f) .

Biotin Conjugation

Biotinylation is a common technique used to label antibodies with biotin, a vitamin that binds with high affinity to streptavidin and avidin. The biotinylated MHC Class II (I-A) mouse antibody can be used in various applications, including flow cytometry, immunohistochemistry, and immunoprecipitation. The biotin-streptavidin interaction allows for the detection and quantification of the antibody-bound MHC class II molecules using streptavidin-conjugated enzymes or fluorophores .

Applications

The MHC Class II (I-A) mouse antibody has several applications in immunological research:

  • Flow Cytometry: Used to analyze the expression of MHC class II molecules on the surface of various cell types.
  • Immunohistochemistry: Used to detect MHC class II molecules in tissue sections, both frozen and paraffin-embedded.
  • Immunoprecipitation: Used to isolate MHC class II molecules from cell lysates for further analysis.
  • Blocking Assays: Used to inhibit MHC class II-mediated antigen presentation to study the role of these molecules in immune responses .

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