MHC CLASS II, I-E Antibody, Biotin
Description
Product Specs
Product Science Overview
The Major Histocompatibility Complex (MHC) Class II molecules are essential components of the immune system. They play a crucial role in the presentation of antigens to T cells, which is vital for initiating immune responses. The MHC Class II molecules are primarily expressed on antigen-presenting cells (APCs) such as B cells, dendritic cells, and macrophages. In mice, the MHC Class II molecules are encoded by the I-A and I-E subregions.
The MHC Class II (I-E) molecules are glycoproteins encoded by the I-E subregion of the MHC complex. These molecules are involved in presenting extracellularly derived peptides to CD4+ T helper cells. The interaction between MHC Class II molecules and T cell receptors (TCRs) is critical for the activation and differentiation of T cells, which subsequently orchestrate various immune responses.
The M5/114.15.2 monoclonal antibody is a widely used reagent for detecting MHC Class II molecules in mice. This antibody specifically reacts with both I-A and I-E subregion-encoded glycoproteins, including I-A^b, I-A^d, I-A^q, I-E^d, and I-E^k, but not with I-A^f, I-A^k, or I-A^s . The M5/114.15.2 antibody is known for its ability to inhibit I-A-restricted T cell responses in certain haplotypes, making it a valuable tool for immunological research .
Biotin is a small molecule that can be conjugated to antibodies to facilitate their detection and purification. Biotinylated antibodies can be easily detected using streptavidin or avidin conjugated to various reporter molecules such as enzymes or fluorophores. This conjugation enhances the versatility of the antibody in various applications, including flow cytometry, immunohistochemistry, and immunoprecipitation .
The MHC Class II (I-E) (Mouse Antibody, Biotin) has a wide range of applications in immunological research:
- Flow Cytometry: Used to analyze the expression of MHC Class II molecules on the surface of cells.
- Immunohistochemistry: Employed to detect MHC Class II molecules in tissue sections.
- Immunoprecipitation: Utilized to isolate MHC Class II molecules from cell lysates.
- Functional Assays: Applied to study the role of MHC Class II molecules in T cell activation and immune responses .
