LY6G Antibody, Biotin

LY6G is a 21-25 kDa protein that is expressed on the majority of myeloid cells in the bone marrow and peripheral granulocytes . It is a cell surface protein that plays a crucial role in the differentiation and maturation of granulocytes. The expression levels of LY6G correlate with granulocyte differentiation, making it a valuable marker for studying these cells .

The Rat Anti-Mouse LY6G Antibody is a monoclonal antibody produced by immunizing rats with LY6G-transfected EL-4J cell lines . This antibody is then purified using affinity chromatography and conjugated with biotin under optimal conditions. The biotinylation of the antibody allows for easy detection and quantification using streptavidin-based detection systems .

The LY6G antibody has several applications in research, particularly in immunology and cell biology. Some of the key applications include:

• Flow Cytometry (FC): The antibody is commonly used for immunofluorescent staining and flow cytometric analysis of granulocytes. It helps in identifying and quantifying LY6G-expressing cells in various samples .
• Immunohistochemistry (IHC): Although not verified in-house, the antibody has been reported in the literature for use in immunohistochemical staining of frozen and paraffin-embedded tissue sections .
• Depletion Studies: The LY6G antibody can be used to deplete granulocytes in vivo, making it useful for studying the role of these cells in various biological processes .

The antibody solution should be stored undiluted between 2°C and 8°C and should not be frozen. Proper storage ensures the stability and effectiveness of the antibody for various applications .