LY6C/G Antibody
LY6C/G, Rat Anti Mouse Antibody
Cat. No.
BT24074
Source
Synonyms
Appearance
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
Product Specs
Formulation
Supplied at a concentration of 1mg/ml in PBS following reconstitution.
Shipping Conditions
The antibody is shipped in a lyophilized format at ambient temperature.
Storage Procedures
For long-term storage, maintain the lyophilized antibody at 4°C in a dry environment. After reconstitution, it is recommended to aliquot the antibody and store it at -20°C if not intended for use within one month.
Solubility
Reconstitute the antibody by adding H20. Gently mix the solution, ensuring to wash the sides of the vial, and allow it to stand for 30-60 seconds before use.
Applications
This antibody is suitable for applications such as flow cytometry, immunohistochemistry, and cell depletion. It exhibits binding affinity for both Ly-6G and Ly-6C (granulocyte receptor 1- Gr-1). Notably, Ly-6G is expressed on the majority of myeloid cells present in the bone marrow and on all peripheral granulocytes. Conversely, Ly-6C demonstrates expression on specific subsets of lymphocytes and monocytes. For optimal results in flow cytometry, it is recommended to use 10 µl of antibody per 10^6 cells. The precise titer required for in vivo cell depletion (cytotoxicity) should be empirically determined by the investigator.
Available Conjugates
In addition to the unconjugated form, this antibody is also offered with biotin and FITC conjugates.
Purification Method
Protein A column.
Type
Rat Anti Mouse Monoclonal.
Clone
YRmLy-6C/G.
Immunogen
BM-enriched myeloid cells.
Ig Subclass
Rat IgG2b.
Product Science Overview
Structure and Function
LY6C/G is a differentiation antigen expressed on several immune cell types, including macrophage/dendritic cell precursors, granulocytes, and certain subpopulations of B- and T-lymphocytes . The protein is approximately 14-17 kDa in size and is involved in various stages of immune cell development and activation .
Applications in Research
The Rat Anti-Mouse LY6C/G antibody is a monoclonal antibody used extensively in research to study the expression and function of LY6C/G in mouse models. This antibody is particularly useful in flow cytometry, immunohistochemistry, and other immunoassays. It helps in identifying and isolating different subsets of immune cells based on their LY6C/G expression levels .
Key Research Findings
- Immune Cell Differentiation: LY6C/G is expressed on macrophage/dendritic cell precursors during mid-stage development. It helps distinguish between different stages of myeloid progenitor cells in mouse bone marrow .
- Inflammation and Immune Response: Immature LY6C monocytes are recruited to sites of acute peripheral inflammation and can develop into tissue macrophages and dendritic cells .
- Memory T Cells: LY6C is also reported to be an indicator of memory CD8+ T cells, which are crucial for long-term immune memory .
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