LY6C/G Antibody, FITC
LY6C/G, Rat Anti Mouse Antibody, FITC
Cat. No.
BT24251
Source
Synonyms
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Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
Product Specs
Formulation
Supplied at 1mg/ml in PBS (after reconstitution).
Shipping Conditions
Antibody is shipped lyophilized at room temperature.
Storage Procedures
Lyophilized antibody can be stored at 4 degrees Celsius in a dry environment for long periods. After reconstitution, aliquot and store at -20 degrees Celsius for future use.
Solubility
Reconstitute the antibody in H2O. Mix the solution gently, wash the sides of the vial and wait for 30-60 seconds before use.
Applications
This antibody can be used in flow cytometry and immunohistochemistry.
This antibody binds to both Ly-6G and Ly-6C (granulocyte receptor 1- Gr-1). Ly-6G is found on most myeloid cells in the bone marrow and on all peripheral granulocytes. Ly-6C is also found on subsets of lymphocytes and monocytes. For flow cytometry applications, use 10 µl of antibody for every 106 cells. The optimal titer for IHC experiments should be determined by the investigator.
This antibody binds to both Ly-6G and Ly-6C (granulocyte receptor 1- Gr-1). Ly-6G is found on most myeloid cells in the bone marrow and on all peripheral granulocytes. Ly-6C is also found on subsets of lymphocytes and monocytes. For flow cytometry applications, use 10 µl of antibody for every 106 cells. The optimal titer for IHC experiments should be determined by the investigator.
Available Conjugates
This antibody is available unconjugated, conjugated to FITC, and conjugated to biotin.
Purification Method
Protein A column.
Type
Rat Anti Mouse Monoclonal.
Clone
YRmLy-6C/G.
Immunogen
BM-enriched myeloid cells.
Ig Subclass
Rat IgG2b.
Product Science Overview
LY6C/G Antigen
The LY6C/G antigen is a glycosylphosphatidylinositol (GPI)-anchored protein found on the surface of certain immune cells. It is expressed on:
- Monocytes and Macrophages: These are crucial components of the innate immune system, responsible for phagocytosis and antigen presentation.
- Granulocytes: These include neutrophils, eosinophils, and basophils, which are involved in inflammatory responses.
- Endothelial Cells: These cells line blood vessels and are involved in the regulation of vascular functions.
- T-cell Subsets: LY6C is expressed on subsets of CD8+ and CD4+ T cells, particularly in certain mouse strains .
Rat Anti-Mouse LY6C/G Antibody
The Rat Anti-Mouse LY6C/G antibody is a monoclonal antibody that specifically binds to the LY6C/G antigen. It is produced by immunizing rats with mouse LY6C/G antigen and then isolating the specific antibody-producing cells. The antibody is then conjugated with FITC, a fluorescent dye, to allow for detection using flow cytometry.
Key Features:
Applications in Research
The FITC-conjugated Rat Anti-Mouse LY6C/G antibody is widely used in immunological research for:
- Flow Cytometry: To analyze the expression of LY6C/G on various cell populations.
- Cell Sorting: To isolate specific cell populations based on LY6C/G expression.
- Immunofluorescence: To visualize LY6C/G-expressing cells under a fluorescence microscope.
Importance in Immunology
The study of LY6C/G expression is important for understanding the roles of different immune cell populations in health and disease. For example:
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